60135

antibody from STEMCELL Technologies Inc

Targeting: FCGR2A CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, IGFR2

Flow cytometry (Recommended by provider)

Antibody Data

Product number

60135

Provider

STEMCELL Technologies Inc

Product name

Anti-Human CD32 Antibody, Clone FLI8.26

Antibody type

Monoclonal

Description

The FLI8.26 antibody reacts with human CD32 (Fc?RII), an ~40 kDa type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, immunomodulation, and platelet aggregation. The FLI8.26 antibody cross-reacts with monocytes, granulocytes, and platelets. CD32 is encoded by three genes (A, B, C) and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. All isoforms are expressed by monocytes/macrophages, placental trophoblasts, and endothelial cells. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. Isoform IIc is expressed by NK cells and neutrophils. Clone FLI8.26 is reactive with both Fc?RIIa and Fc?RIIb and inhibits binding of the Fc?RIIa-specific antibody, clone IV.3, in flow cytometry analyses, suggesting that these clones may share a common or overlapping epitope on Fc?RIIa. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes. These interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the FLI8.26 antibody may be used as a blocking antibody to reduce non-specific binding.

Reactivity

Human

Isotype

IgG

Vial size

100 µg

Storage

2 - 8°C