LS-C797116

antibody from LSBio

Targeting: FCGR2A CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, IGFR2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C797116

Provider

LSBio

Product name

CD32A Antibody (clone OTI9C6, Carrier-free) LS-C797116

Antibody type

Monoclonal

Description

Affinity chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI9C6

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded breast tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded colon tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Kidney tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded lung tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded breast tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded colon tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Kidney tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded lung tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded prostate tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded colon tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Kidney tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).