AF1875

antibody from R&D Systems

Targeting: FCGR2A CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, IGFR2

Western blot (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF1875

Provider

R&D Systems

Product name

Human Fc gamma RIIA/CD32a Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Fc gamma RIIA/CD32a in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Fc gamma RIIB and rhFc gamma RIIIB is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

AAA35827


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Antibody-dependent and antibody-independent uptake of HBsAg across human leucocyte subsets is similar between individuals with chronic hepatitis B virus infection and healthy donors.

Tharinger H, Rebbapragada I, Samuel D, Novikov N, Nguyen MH, Jordan R, Frey CR, Pflanz S
Journal of viral hepatitis 2017 Jun;24(6):506-513

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Viremic HIV Controllers Exhibit High Plasmacytoid Dendritic Cell-Reactive Opsonophagocytic IgG Antibody Responses against HIV-1 p24 Associated with Greater Antibody Isotype Diversification.

Tjiam MC, Taylor JP, Morshidi MA, Sariputra L, Burrows S, Martin JN, Deeks SG, Tan DB, Lee S, Fernandez S, French MA
Journal of immunology (Baltimore, Md. : 1950) 2015 Jun 1;194(11):5320-8

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FcγRI mediates serum amyloid P inhibition of fibrocyte differentiation.

Crawford JR, Pilling D, Gomer RH
Journal of leukocyte biology 2012 Oct;92(4):699-711

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Nonfucosylated anti-CD20 antibody potentially induces apoptosis in lymphoma cells through enhanced interaction with FcgammaRIIIb on neutrophils.

Nakagawa T, Natsume A, Satoh M, Niwa R
Leukemia research 2010 May;34(5):666-71

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Association of FcgammaRIIa (CD32a) with lipid rafts regulates ligand binding activity.

Bournazos S, Hart SP, Chamberlain LH, Glennie MJ, Dransfield I
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):8026-36

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The ubiquitin ligase c-Cbl down-regulates FcgammaRIIa activation in human neutrophils.

Marois L, Vaillancourt M, Marois S, Proulx S, Paré G, Rollet-Labelle E, Naccache PH
Journal of immunology (Baltimore, Md. : 1950) 2009 Feb 15;182(4):2374-84

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C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.

Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
Journal of leukocyte biology 2008 Oct;84(4):1109-19

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C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque.

Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M
Atherosclerosis 2008 Jan;196(1):129-35

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C-reactive protein impairs human CD14+ monocyte-derived dendritic cell differentiation, maturation and function.

Zhang R, Becnel L, Li M, Chen C, Yao Q
European journal of immunology 2006 Nov;36(11):2993-3006