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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-52739 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAM163A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YYCCKKSGTE VADEEEEREH DLPTHPRGPT CNACSSQALD GRGSLAPLTS EPCSQPCGVA ASHCTTCSPY SSPFYIRTAD MVPNGGGGER LSFAPTYYKE GGPPSLKLAA PQSYPVTWPG SGREAFTNPR AIST
- Concentration
- 0.05 mg/mL
Submitted references The Role of Aberrant DNA Methylation in Misregulation of Gene Expression in Gonadotroph Nonfunctioning Pituitary Tumors.
Kober P, Boresowicz J, Rusetska N, Maksymowicz M, Paziewska A, Dąbrowska M, Kunicki J, Bonicki W, Ostrowski J, Siedlecki JA, Bujko M
Cancers 2019 Oct 25;11(11)
Cancers 2019 Oct 25;11(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FAM163A in control (vector only transfected HEK293T lysate) and FAM163A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a FAM163A Polyclonal Antibody (Product # PA5-52739).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FAM163A in human adrenal gland tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using a FAM163A Polyclonal Antibody (Product # PA5-52739).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Difference in DNA methylation at regulatory regions, and expression levels of HIF3A , FAM163A , and PRSS8 in gonadotroph NFPA samples and normal pituitary. ( A ) Pyrosequencing analysis of CpG sites. Each dot represents the methylation level in a particular sample. Mean values are shown as horizontal lines. ( B ) Relative expression levels of HIF3A , FAM163A , and PRSS8 in normal pituitary and NFPAs. Each dot represents the expression level in the sample. Mean values are shown as horizontal lines. ( C ) Examples of immunohistochemical staining of normal pituitary and NFPAs with antibodies against HIF3A, FAM163A, and prostasin (PRSS8). Magnification, x400.
