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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- HPA040741 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA040741, RRID:AB_10794859
- Product name
- Anti-LEO1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human LEO1, Gene description: Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae), Validated applications: ICC, IHC, WB, Uniprot ID: Q8WVC0, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references SARS-CoV-2 variants evolve convergent strategies to remodel the host response
Bouhaddou M, Reuschl A, Polacco B, Thorne L, Ummadi M, Ye C, Rosales R, Pelin A, Batra J, Jang G, Xu J, Moen J, Richards A, Zhou Y, Harjai B, Stevenson E, Rojc A, Ragazzini R, Whelan M, Furnon W, De Lorenzo G, Cowton V, Syed A, Ciling A, Deutsch N, Pirak D, Dowgier G, Mesner D, Turner J, McGovern B, Rodriguez M, Leiva-Rebollo R, Dunham A, Zhong X, Eckhardt M, Fossati A, Liotta N, Kehrer T, Cupic A, Rutkowska M, Mena I, Aslam S, Hoffert A, Foussard H, Olwal C, Huang W, Zwaka T, Pham J, Lyons M, Donohue L, Griffin A, Nugent R, Holden K, Deans R, Aviles P, Lopez-Martin J, Jimeno J, Obernier K, Fabius J, Soucheray M, Hüttenhain R, Jungreis I, Kellis M, Echeverria I, Verba K, Bonfanti P, Beltrao P, Sharan R, Doudna J, Martinez-Sobrido L, Patel A, Palmarini M, Miorin L, White K, Swaney D, Garcia-Sastre A, Jolly C, Zuliani-Alvarez L, Towers G, Krogan N
Cell 2023;186(21):4597-4614.e26
Cell 2023;186(21):4597-4614.e26
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and LEO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403376).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center & centrosome.
- Sample type
- Human
