- A300-175A - Invitrogen Antibodies LEO1 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Detection of mouse Leo1 by western blot. Samples: Whole cell lysate (15 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Leo1 antibody (Product # A300-175A; lot A300-175A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of human Leo1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, MCF-7, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Leo1 antibody (Product # A300-175A; lot A300-175A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of mouse Leo1 by western blot. Samples: Whole cell lysate (50 µg) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Leo1 antibody A300-175A (lot A300-175A-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of human Leo1 by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL; 10, 30 or 50 µg) from control 293T cells (E) or 293T cells transfected with a Leo1 expression construct (T). B. Whole cell lysate (10mg) from 293T cells. Antibodies: Affinity purified rabbit anti-Leo1 antibody BL677 (Cat. No. A300-175A) used at 1 µg/ml for western blot in A and B. In B, Leo1 was immunoprecipitated by using BL677 at 3 µg/10mg with or without blocking peptide. Detection: Chemiluminescence with an exposure time of 1 minute. *The identity of the upper band is unknown but may represent a modified form of Leo1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of human Leo1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Leo1 (Product # A300-175A; lot 3) used at 1:5,000 (0.2 µg/mL). Detection: DAB.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of mouse Leo1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Leo1 (Product # A300-175A lot 3) used at 1:5,000 (0.2 µg/mL). Detection: DAB.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of human and mouse Leo1 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-LEO1 (Cat. No. A300-175A Lot1) used at a dilution of 1:5,000 (0.2µg/ml) and 1:1,000 (1µg/ml). Detection: DAB.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of human Leo1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Leo1 antibody (Product # A300-175A; lot A300-175A-3) used for IP at 6 µg per reaction. Leo1 was also immunoprecipitated by a previous lot of this antibody (lot A300-175A-2). For blotting immunoprecipitated Leo1, A300-175A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.

A300-175A