PA5-121012

antibody from Invitrogen Antibodies

Targeting: CST1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-121012

Provider

Invitrogen Antibodies

Product name

CST1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: A-431, A-549, BT-474, THP-1. The target is usually found in the following locations: Secreted. Immunogen sequence: AWSPKEEDRI IPGGIYNADL NDEWVQRALH FAISEYNKAT KDDYYRRPLR VLRARQQTVG GVNYFFDVEV GRTICTKSQP NLDTCAFHEQ PELQKKQLCS FEIYEVPWEN RRSLVKSRCQ ES

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

3.75 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CST1 in various cell lines using a CST1 Polyclonal antibody (Product # PA5-121012) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 30s.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CST1 in human prostate using a Polyclonal antibody (Product # PA5-121012) at a dilution of 1:100.