PA5-21541

antibody from Invitrogen Antibodies

Targeting: GALNT2 GalNAc-T2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21541

Provider

Invitrogen Antibodies

Product name

GALNT2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Molt-4, mouse adipose, rat brain. Predicted reactivity: Mouse (97%), Rat (98%), Zebrafish (92%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.77 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery.

Khakurel A, Kudlyk T, Bonifacino JS, Lupashin VV
Molecular biology of the cell 2021 Aug 15;32(17):1594-1610

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TLR7 in B cells promotes renal inflammation and Gd-IgA1 synthesis in IgA nephropathy.

Zheng N, Xie K, Ye H, Dong Y, Wang B, Luo N, Fan J, Tan J, Chen W, Yu X
JCI insight 2020 Jul 23;5(14)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GALNT2 showing staining in the Golgi apparatus of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a GALNT2 polyclonal antibody (Product # PA5-21541) diluted at 1:500. Blue: Hoechst 33343 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

GALNT2 Polyclonal Antibody detects GALNT2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GALNT2 stained by GALNT2 Polyclonal Antibody (Product # PA5-21541) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 3: GARP-KO affects the stability of key N- and O- Golgi glycosylation enzymes in RPE1 and HeLa cells. (A-C) WB (top panel) and quantification (bottom panels) of MGAT1 (A), B4GalT1 (B), and GalNacT2 (C) in WT, VPS53-KO, VPS54-KO, and the corresponding rescued RPE1 cells. (D) WT, VPS53-KO, and VPS53-KO rescued RPE1 cells were co-stained for endogenous B4GalT1 (green) and GM130 (magenta), and images were taken (left panel). Colocalization of B4GalT1 with GM130 was determined by calculation of the Pearson's correlation coefficient (right panel). At least 30 cells were imaged per sample for the quantification. Each dot in the bar graph (right panel) represents the colocalization of GM130 and B4GalT1 in several (1 to 10) cells imaged per field. (E) WB (top panel) and quantification (bottom panels) of ST6Gal1 in WT, VPS53-KO, VPS54-KO, and the corresponding rescued RPE1 cells. For quantification of ST6Gal1 blot, the additional low molecular weight band in VPS53-KO cells was not included. (F, G) WB (top panel) and quantification (bottom panels) of MGAT1 (F) and B4GalT1 (G) in WT, VPS50-, VPS51-, VPS52-, VPS53-, and VPS54-KO HeLa cells. Values in bar graphs represent the mean +- SD from three independent experiments. Statistical significance was calculated using one-way ANOVA. **** P