AM33441PU-N

antibody from Acris Antibodies GmbH

Targeting: CDNF ARMETL1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM33441PU-N

Provider

Acris Antibodies GmbH

Product name

anti CDNF

Antibody type

Monoclonal

Antigen

Recombinant Human CDNF produced using CHO-based. Antigen is purified from cell culture supernatant.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

7G5

Vial size

0.1 mg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot testing of anti-CDNF monoclonal antibody (6G5). Line 1: PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2: Recombinant CDNF expressed into the supernatant of CHO cell culture medium.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence detection of human CDNF expressed in U2OS cells. CDNF was visualized using anti-CDNF antibody clone 6G5 at 1 μg/ml. Goat ant-mouse AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for CDNF specific signal and blue for nuclei. A. CDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry testing of anti-CDNF monoclonal antibody 6G5. Analysis was performed using formalin-fixed paraffin-embedded human cerebral cortex tissue sections from Alzheimer’s disease patients. Tissue sections were boiled with sodium citrate buffer (pH 6) for antigen retrieval. Incubation with primary antibody at 5 μg/ml was performed overnight at 4°C. DAKO EnVision^TM Detection System, Peroxidase/DAB was used for visualization. Sections were counterstained with toluidine blue and mounted with Eukitt mounting medium. <strong>A.</strong> CDNF staining by monoclonal antibody 6G5; <strong>B.</strong> Negative staining without primary antibody.