PA5-59392

antibody from Invitrogen Antibodies

Targeting: ACSS1 ACAS2L, AceCS2L, dJ568C11.3, MGC33843

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-59392

Provider

Invitrogen Antibodies

Product name

ACSS1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: IFAGFSAESL AGRINDAKCK VVITFNQGLR GGRVVELKKI VDEAVKHCPT VQHVLVAHRT DNKVHMGDLD VPLEQEMAKE DPVCAPESMG SEDMLFML Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 90%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.4 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Acetyl-CoA synthetase 3 promotes bladder cancer cell growth under metabolic stress.

Zhang J, Duan H, Feng Z, Han X, Gu C
Oncogenesis 2020 May 12;9(5):46

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ACSS1 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4. Samples were probed using an ACSS1 Polyclonal Antibody (Product # PA5-59392).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ACSS1 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4. Samples were probed using an ACSS1 Polyclonal Antibody (Product # PA5-59392).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 ACSS3 is induced under metabolic stress and facilitate BLCA cell growth. a ACSS1, ACSS2, and ACSS3 mRNA expression levels in BLCA cells under normoxia (21% O 2 ) or hypoxia (1% O 2 ) culture with 10% or 1% serum levels. b ACSS1, ACSS2, and ACSS3 protein expression levels in BLCA cells under normoxia (21% O 2 ) or hypoxia (1% O 2 ) culture with 10% or 1% serum levels. c ACSS1, ACSS2, and ACSS3 protein expression levels in BLCA cells transfected with control of ACSS3 siRNA. d Colony formed from BLCA cells transfected with control of ACSS3 siRNA under indicated culture situations. e Cell number of BLCA cells transfected with control of ACSS3 siRNA under indicated culture situations after 48 h incubation. f Percentage of lipogenic AcCoA generation from acetate (labeled by 13 C) in BLCA cells transfected with control of ACSS3 siRNA under hypoxia (1% O 2 ) culture. g Percentage of lipogenic AcCoA generation from glutamine or glucose (labeled by 13 C) in BLCA cells transfected with control of ACSS3 siRNA under hypoxia (1% O 2 ) culture. h Subcellular localization of ACSS3-Myc expressed in T24 cells. ACSS3 was detected with anti-myc antibody. Mitochondria was stained with MitoTracker.