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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF7747 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal ST6 Gal Sialyltransferase 2/ST6GAL2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human ST6 Gal Sialyltransferase 2/ST6GAL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ST6GAL1 is observed.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model.
Identification of target proteins involved in cochlear otosclerosis.
Karhadkar TR, Pilling D, Cox N, Gomer RH
Scientific reports 2017 Nov 8;7(1):15069
Scientific reports 2017 Nov 8;7(1):15069
Identification of target proteins involved in cochlear otosclerosis.
Richard C, Doherty JK, Fayad JN, Cordero A, Linthicum FH Jr
Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology 2015 Jun;36(5):923-31
Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology 2015 Jun;36(5):923-31
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human ST6 Gal Sialyltransferase 2/ST6GAL2 by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7747) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST6 Gal Sialyltransferase 2/ST6GAL2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ST6 Gal Sialyltransferase 2/ST6GAL2 in Human Brain. ST6 Gal Sialyltransferase 2/ST6GAL2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7747) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
