Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-67257 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KIAA0319L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KRKSKYKILDA TDQESLELKP TSRAGIKQKG LLLSSSLMHS ESELDSDDAI FTWPDREKGK LLHGQNGSVP NGQ Highest antigen sequence identity to the following orthologs - mouse 93%, rat 93%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Identification of AAV serotypes for lung gene therapy in human embryonic stem cell-derived lung organoids.
Meyer-Berg H, Zhou Yang L, Pilar de Lucas M, Zambrano A, Hyde SC, Gill DR
Stem cell research & therapy 2020 Oct 23;11(1):448
Stem cell research & therapy 2020 Oct 23;11(1):448
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of KIAA0319L in human cell line U-2 OS shows localization to the Golgi apparatus. Samples were probed using a KIAA0319L Polyclonal Antibody (Product # PA5-67257).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Immunohistochemistry for markers of alveolar type II cells and AAV entry receptors. Representative images are shown of fixed-frozen sections of LBOs ( n = 2-3), with nuclei stained with DAPI (blue) and various markers (red), including ATII cell marker SP-C ( a ), ATII cell marker SP-B ( b , c ), universal AAV co-receptor AAVR ( d ), glycan receptor alpha-2,3-linked sialic acid ( e , f ) and glycan receptor heparan sulphate ( g , h ). Native fluorescence is observed following microinjection of AAV6.2-CMV-eGFP ( c ) compared with negative control LBOs ( b ). Sections digested with sialidase A ( e ) and heparinase III ( g ) to remove glycans are included as negative staining controls. Staining controls for images a - d are shown in suppl. Fig. 2 . Scale bar 50 mum
