Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- HPA015914 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA015914, RRID:AB_1846794
- Product name
- Anti-CISD2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human CISD2, Gene description: CDGSH iron sulfur domain 2, Alternative Gene Names: ERIS, Miner1, NAF-1, WFS2, ZCD2, Validated applications: WB, IHC, ICC, Uniprot ID: Q8N5K1, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Upregulation of CISD2 augments ROS homeostasis and contributes to tumorigenesis and poor prognosis of lung adenocarcinoma
Breast cancer tumorigenicity is dependent on high expression levels of NAF-1 and the lability of its Fe-S clusters
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Li S, Chen C, Chen Y, Yen Y, Fang W, Tsai F, Chang J, Shen Y, Huang S, Chuu C, Chang I, Hsiung C, Jiang S
Scientific Reports 2017;7(1)
Scientific Reports 2017;7(1)
Breast cancer tumorigenicity is dependent on high expression levels of NAF-1 and the lability of its Fe-S clusters
Darash-Yahana M, Pozniak Y, Lu M, Sohn Y, Karmi O, Tamir S, Bai F, Song L, Jennings P, Pikarsky E, Geiger T, Onuchic J, Mittler R, Nechushtai R
Proceedings of the National Academy of Sciences 2016;113(39):10890-10895
Proceedings of the National Academy of Sciences 2016;113(39):10890-10895
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323
Nature Methods 2013;10(4):315-323
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and CISD2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423415).
- Sample type
- Human
- Protocol
- Protocol