55054-1-AP

antibody from Proteintech Group

Targeting: USP9X DFFRX, FAF, MRX99

Provider product page for 55054-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [50]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 55054-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#55054-1-AP, RRID:AB_10792932
Product name: USP9X antibody
Antibody type: Polyclonal
Description: KD/KO validated USP9X antibody (Cat. #55054-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

USP9X protein structure - 55054-1-AP shown in red.

Submitted references USP9X promotes acute myeloid leukemia progression and adriamycin resistance by regulating METTL7A deubiquitination.

Yan Z, Siyi W, Siyu W, YuanYuan T, Jiajia L
Cellular signalling 2026 Mar;139:112314

NDR1 enhances USP9X-mediated AR deubiquitination and promotes enzalutamide resistance in castration-resistant prostate cancer.

Zheng Z, Li J, Du Y, Li L, Wu Q, Liu B, Wu H, Zhang Z, Xuan Z, Zhao Y, Sun H, Shao C
International journal of biological sciences 2025;21(13):5628-5644

Rescue of ciliogenesis and hyperglutamylation mutant phenotype in AGBL5(-/-) cell model of retinitis pigmentosa.

Villa-Vasquez SS, Nazlamova L, Pengelly RJ, Wilson DI, Baralle D, Wheway G
BMC molecular and cell biology 2025 Sep 9;26(1):27

Rictor stability mediated by USP9X regulates embryo implantation by participating in lipid metabolism of endometrium†.

Peng M, He J, Liu X, Mu X, Yin X, Liu T, Chen X, Gao R, Wang Y, Feng Q, Geng Y
Biology of reproduction 2025 Sep 15;113(3):642-656

LZTR1 is a melanoma oncogene that promotes invasion and suppresses apoptosis.

Bacchiocchi A, Mak M, Khan ZM, Gong X, Sznol M, Na Z, Su H, Chan LH, Yan Q, Zhao D, Mortlock RD, Knight J, Slavoff SA, Halaban R
Oncogene 2025 Oct;44(41):3974-3984

Deubiquitinase USP9X controls Wnt signaling for CNS vascular formation and barrier maintenance.

Lei Y, Hu J, Zhao J, Liu Q, Zhang SW, Wu F, Liu Y, Ren H, Qin X, Wu X, Gao F, Hu J, Ouyang K, Liu Q, Zheng X, Shi L, Wang X
Developmental cell 2025 Jun 9;60(11):1618-1635.e7

Ubiquitin Specific Protease 9X Regulates the Activation of ARK5 and Promotes Progression of Fibrotic Remodeling.

Li X, Jiang S, Yang W, Zhu X, Zhang F, Li Z, Guo X, Wei Y
JACC. Basic to translational science 2025 Jul;10(7):101255

Sirt6, Deubiquitinated and Stabilised by USP9X, Takes Essential Actions on the Pathogenesis of Experimental Autoimmune Myasthenia Gravis by Regulating CD4(+) T Cells.

Xie C, Zhang HL, Yuan J, Zhang Y, Liu YC, Xu Q, Chen LR
Clinical and experimental pharmacology & physiology 2025 Feb;52(2):e70018

MLKL-USP7-UBA52 signaling is indispensable for autophagy in brain through maintaining ubiquitin homeostasis.

Zhang Z, Chen S, Jun S, Xu X, Hong Y, Yang X, Zou L, Song YQ, Chen Y, Tu J
Autophagy 2025 Feb;21(2):424-446

Deletion of Xist repeat B disrupts cell cycle and asymmetric cell division through Usp9x hyperactivation in mice.

Liang M, Zhang L, Gong H, Yang L, Wang H, Song N, Lai L, Xie W, Li Z
Nucleic acids research 2025 Feb 27;53(5)

USP9X integrates TGF-β and hypoxia signalings to promote ovarian cancer chemoresistance via HIF-2α-maintained stemness.

Zhang Z, Yu X, Wen L, Wang J, Li Z, Zhang Y, Cheng J, Kan R, Zhang W, Shen Y, Yuan S, Zhao L
Cell death & disease 2025 Apr 18;16(1):312

USP9X-mediated deubiquitination of Raptor contributes to autophagy impairment and memory deficits in P301S mice.

Zheng S, Zhu J, Wang C, Wu Y, Sun S, Guo H, Chang Y, Ma R, Li G
Cell communication and signaling : CCS 2024 Oct 24;22(1):516

USP9X-mediated REV1 deubiquitination promotes lung cancer radioresistance via the action of REV1 as a Rad18 molecular scaffold for cystathionine γ-lyase.

Chen Y, Feng X, Wu Z, Yang Y, Rao X, Meng R, Zhang S, Dong X, Xu S, Wu G, Jie X
Journal of biomedical science 2024 May 28;31(1):55

Crym-positive striatal astrocytes gate perseverative behaviour.

Ollivier M, Soto JS, Linker KE, Moye SL, Jami-Alahmadi Y, Jones AE, Divakaruni AS, Kawaguchi R, Wohlschlegel JA, Khakh BS
Nature 2024 Mar;627(8003):358-366

USP46 enhances tamoxifen resistance in breast cancer cells by stabilizing PTBP1 to facilitate glycolysis.

Gao S, Wang Y, Xu Y, Liu L, Liu S
Biochimica et biophysica acta. Molecular basis of disease 2024 Mar;1870(3):167011

USP9X regulates the proliferation, survival, migration and invasion of gastric cancer cells by stabilizing MTH1.

Xu W, Zhang Y, Su Y, Li L, Yang X, Wang L, Gao H
BMC gastroenterology 2024 Jul 29;24(1):239

Deubiquitinase USP9x regulates the proline biosynthesis pathway in non-small cell lung cancer.

Becirovic T, Zhang B, Lindskog C, Norberg E, Vakifahmetoglu-Norberg H, Kaminskyy VO, Kochetkova E
Cell death discovery 2024 Jul 29;10(1):342

Pharmaceutical targeting of OTUB2 sensitizes tumors to cytotoxic T cells via degradation of PD-L1.

Ren W, Xu Z, Chang Y, Ju F, Wu H, Liang Z, Zhao M, Wang N, Lin Y, Xu C, Chen S, Rao Y, Lin C, Yang J, Liu P, Zhang J, Huang C, Xia N
Nature communications 2024 Jan 2;15(1):9

CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2 Degradation via Interacting with UCHL3.

Xie G, Lei B, Yin Z, Xu F, Liu X
International journal of molecular sciences 2024 Feb 29;25(5)

Avenanthramide A potentiates Bim-mediated antineoplastic properties of 5-fluorouracil via targeting KDM4C/MIR17HG/GSK-3β negative feedback loop in colorectal cancer.

Fu R, Dou Z, Li N, Fan X, Amin S, Zhang J, Wang Y, Li Z, Li Z, Yang P
Acta pharmaceutica Sinica. B 2024 Dec;14(12):5321-5340

IGFALS suppresses hepatocellular carcinoma progression by stabilizing PPAR-γ.

Xu L, Xiong L, Chen Y, Chen J, Liu X, Xu Y, Shen Y, Wang S, Yu S, Xu X
International immunopharmacology 2024 Dec 25;143(Pt 2):113414

The deubiquitinase USP9X regulates RIT1 protein abundance and oncogenic phenotypes.

Riley AK, Grant M, Snell A, Cromwell E, Vichas A, Moorthi S, Rominger C, Modukuri SP, Urisman A, Castel P, Wan L, Berger AH
iScience 2024 Aug 16;27(8):110499

USP9X deubiquitinates and stabilizes CDC123 to promote breast carcinogenesis through regulating cell cycle.

Song N, Deng L, Zeng L, He L, Liu C, Liu L, Fu R
Molecular carcinogenesis 2023 Oct;62(10):1487-1503

BioE3 identifies specific substrates of ubiquitin E3 ligases.

Barroso-Gomila O, Merino-Cacho L, Muratore V, Perez C, Taibi V, Maspero E, Azkargorta M, Iloro I, Trulsson F, Vertegaal ACO, Mayor U, Elortza F, Polo S, Barrio R, Sutherland JD
Nature communications 2023 Nov 23;14(1):7656

Ablation of EWS-FLI1 by USP9X inhibition suppresses cancer cell growth in Ewing sarcoma.

Wang S, Huo X, Yang Y, Mo Y, Kollipara RK, Kittler R
Cancer letters 2023 Jan 1;552:215984

The deubiquitinase USP9X regulates RIT1 protein abundance and oncogenic phenotypes.

Riley AK, Grant M, Snell A, Vichas A, Moorthi S, Urisman A, Castel P, Wan L, Berger AH
bioRxiv : the preprint server for biology 2023 Dec 1;

Goliath induces inflammation in obese mice by linking fatty acid β-oxidation to glycolysis.

Hao S, Zhang S, Ye J, Chen L, Wang Y, Pei S, Zhu Q, Xu J, Tao Y, Zhou N, Yin H, Duan CW, Mao C, Zheng M, Xiao Y
EMBO reports 2023 Apr 5;24(4):e56932

Targeting USP9X-AMPK Axis in ARID1A-Deficient Hepatocellular Carcinoma.

Zhang FK, Ni QZ, Wang K, Cao HJ, Guan DX, Zhang EB, Ma N, Wang YK, Zheng QW, Xu S, Zhu B, Chen TW, Xia J, Qiu XS, Ding XF, Jiang H, Qiu L, Wang X, Chen W, Cheng SQ, Xie D, Li JJ
Cellular and molecular gastroenterology and hepatology 2022;14(1):101-127

Induction of zinc finger protein RNF6 auto-ubiquitination for the treatment of myeloma and chronic myeloid leukemia.

Zhuang H, Ren Y, Mao C, Zhong Y, Zhang Z, Cao B, Zhang Y, Huang J, Xu G, Huang Z, Xu Y, Mao X
The Journal of biological chemistry 2022 Sep;298(9):102314

USP15 regulates p66Shc stability associated with Drp1 activation in liver ischemia/reperfusion.

Tian X, Zhao Y, Yang Z, Lu Q, Zhou L, Zheng S
Cell death & disease 2022 Sep 26;13(9):823

Deubiquitinating enzyme USP9X regulates metastasis and chemoresistance in triple-negative breast cancer by stabilizing Snail1.

Guan T, Yang X, Liang H, Chen J, Chen Y, Zhu Y, Liu T
Journal of cellular physiology 2022 Jul;237(7):2992-3000

TGFBR2 is a novel substrate and indirect transcription target of deubiquitylase USP9X in granulosa cells.

Yang L, Wang S, Pan Z, Du X, Li Q
Journal of cellular physiology 2022 Jul;237(7):2969-2979

A Proteomic Approach for Systematic Mapping of Substrates of Human Deubiquitinating Enzymes.

Ramirez J, Prieto G, Olazabal-Herrero A, Borràs E, Fernandez-Vigo E, Alduntzin U, Osinalde N, Beaskoetxea J, Lectez B, Aloria K, Rodriguez JA, Paradela A, Sabidó E, Muñoz J, Corrales F, Arizmendi JM, Mayor U
International journal of molecular sciences 2021 May 3;22(9)

Knockdown of USP9X reverses cisplatin resistance by decreasing β-catenin expression in nasopharyngeal carcinoma cells.

Lu WX, Gan GS, Yang B
Neoplasma 2021 Jul;68(4):810-822

USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription.

Jie X, Fong WP, Zhou R, Zhao Y, Zhao Y, Meng R, Zhang S, Dong X, Zhang T, Yang K, Wu G, Xu S
Cell death and differentiation 2021 Jul;28(7):2095-2111

Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.

Vichas A, Riley AK, Nkinsi NT, Kamlapurkar S, Parrish PCR, Lo A, Duke F, Chen J, Fung I, Watson J, Rees M, Gabel AM, Thomas JD, Bradley RK, Lee JK, Hatch EM, Baine MK, Rekhtman N, Ladanyi M, Piccioni F, Berger AH
Nature communications 2021 Aug 9;12(1):4789

Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.

Dietachmayr M, Rathakrishnan A, Karpiuk O, von Zweydorf F, Engleitner T, Fernández-Sáiz V, Schenk P, Ueffing M, Rad R, Eilers M, Gloeckner CJ, Clemm von Hohenberg K, Bassermann F
Nature communications 2020 Mar 9;11(1):1268

Arginine methylation-dependent LSD1 stability promotes invasion and metastasis of breast cancer.

Liu J, Feng J, Li L, Lin L, Ji J, Lin C, Liu L, Zhang N, Duan D, Li Z, Huang B, Zhang Y, Lu J
EMBO reports 2020 Feb 5;21(2):e48597

Inhibition of USP9X Downregulates JAK2-V617F and Induces Apoptosis Synergistically with BH3 Mimetics Preferentially in Ruxolitinib-Persistent JAK2-V617F-Positive Leukemic Cells.

Akiyama H, Umezawa Y, Watanabe D, Okada K, Ishida S, Nogami A, Miura O
Cancers 2020 Feb 10;12(2)

Detailed Dissection of UBE3A-Mediated DDI1 Ubiquitination.

Elu N, Osinalde N, Beaskoetxea J, Ramirez J, Lectez B, Aloria K, Rodriguez JA, Arizmendi JM, Mayor U
Frontiers in physiology 2019;10:534

Inhibition of the deubiquitinase USP9x induces pre-B cell homeobox 1 (PBX1) degradation and thereby stimulates prostate cancer cell apoptosis.

Liu Y, Xu X, Lin P, He Y, Zhang Y, Cao B, Zhang Z, Sethi G, Liu J, Zhou X, Mao X
The Journal of biological chemistry 2019 Mar 22;294(12):4572-4582

Deubiquitinase USP9X regulates the invasion of prostate cancer cells by regulating the ERK pathway and mitochondrial dynamics.

Zhang J, Wang J, Luan T, Zuo Y, Chen J, Zhang H, Ye Z, Wang H, Hai B
Oncology reports 2019 Jun;41(6):3292-3304

USP9X-mediated deubiquitination of B-cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis.

Shang Z, Zhao J, Zhang Q, Cao C, Tian S, Zhang K, Liu L, Shi L, Yu N, Yang S
The Journal of biological chemistry 2019 Jun 21;294(25):9844-9857

The deubiquitinase USP9X promotes tumor cell survival and confers chemoresistance through YAP1 stabilization.

Li L, Liu T, Li Y, Wu C, Luo K, Yin Y, Chen Y, Nowsheen S, Wu J, Lou Z, Yuan J
Oncogene 2018 May;37(18):2422-2431

Deubiquitinating enzyme USP9X regulates cellular clock function by modulating the ubiquitination and degradation of a core circadian protein BMAL1.

Zhang Y, Duan C, Yang J, Chen S, Liu Q, Zhou L, Huang Z, Xu Y, Xu G
The Biochemical journal 2018 Apr 30;475(8):1507-1522

USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability.

Skowyra A, Allan LA, Saurin AT, Clarke PR
Cell reports 2018 Apr 17;23(3):852-865

Deubiquitinating Enzyme USP9X Suppresses Tumor Growth via LATS Kinase and Core Components of the Hippo Pathway.

Toloczko A, Guo F, Yuen HF, Wen Q, Wood SA, Ong YS, Chan PY, Shaik AA, Gunaratne J, Dunne MJ, Hong W, Chan SW
Cancer research 2017 Sep 15;77(18):4921-4933

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Li X, Song N, Liu L, Liu X, Ding X, Song X, Yang S, Shan L, Zhou X, Su D, Wang Y, Zhang Q, Cao C, Ma S, Yu N, Yang F, Wang Y, Yao Z, Shang Y, Shi L
Nature communications 2017 Mar 31;8:14866

The X-linked deubiquitinase USP9X is an integral component of centrosome.

Wang Q, Tang Y, Xu Y, Xu S, Jiang Y, Dong Q, Zhou Y, Ge W
The Journal of biological chemistry 2017 Aug 4;292(31):12874-12884

AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells.

Huang KP, Chen C, Hao J, Huang JY, Liu PQ, Huang HQ
Endocrinology 2015 Jan;156(1):268-79

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 55054-1-AP(USP9X antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The USP9X antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human USP9X. This antibody recognizes human,mouse,rat antigen. The USP9X antibody has been validated for the following applications: ELISA, WB, IF, IHC analysis.
Appendix: 55054-1-AP.pdf

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