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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- A305-801A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAM134A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human FAM134A by western blot. Samples: Whole cell lysate (10 µg) from K-562, GaMG, Hep-G2, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FAM134A antibody (Product # A305-801A-T lot 2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human FAM134A by western blot. Samples: Whole cell lysate (50 µg) from K-562, Hep-G2, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FAM134A antibody A305-801A-M (lot A305-801A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human FAM134A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-FAM134A antibody (Product # A305-800A-T; lot 2) used for IP at 20 µL/mg lysate. FAM134A was also immunoprecipitated by a previous lot of this antibody (Product # A305-800A-T; lot 1). For blotting immunoprecipitated FAM134A, Product # A305-801A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
