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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- PA5-37853 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Contactin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine and porcine based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Contactin 1 by a Contactin 1 monoclonal antibody (Product # PA5-37853) at a concentration of 1 µg/mL. Mouse (A) and Rat (B) Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Contactin 1/CNTN1 in human cerebellum lysate was performed using 35 µg protein in RIPA buffer. Lysates were probed with a Contactin 1/CNTN1 polyclonal antibody (Product # PA5-37853) at a dilution of 1 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Contactin 1 in Mouse Cerebral Cortex using a Contactin 1 monoclonal antibody (Product #PA5-37853) at 5 µg/mL. The Mouse Cerebral Cortex tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, DAB-staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Contactin 1 / CNTN1 in paraffin embedded Mouse Cerebral Cortex tissue. Steamed antigen retrieval was performed with citrate buffer pH 6, followed by application of the Contactin 1 / CNTN1 antibody (Product # PA5-37853) (5µg / ml) and DAB-staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Contactin 1/CNTN1 in paraffin-embedded human cerebellum following steamed antigen retrieval with a citrate buffer, pH 6, using a Contactin 1/CNTN1 polyclonal antibody (Product # PA5-37853) at a dilution of 5 µg/mL. Detection was performed using an AP-conjugated secondary antibody.
