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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- R35512 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GP135 Antibody / Contactin 1
- Antibody type
- Polyclonal
- Description
- This highly specific GP135 antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the GP135 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human cerebellum lysate with GP135 antibody at 1ug/ml. Predicted molecular weight: ~113/135-150kDa (unmodified/glycosylated).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) mouse brain and 2) rat brain lysate with GP135 antibody at 1ug/ml. Predicted molecular weight: ~113/135-150kDa (unmodified/glycosylated).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human cerebellum with GP135 antibody at 5ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse cerebral cortex with GP135 antibody at 5ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
