Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF904 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Contactin-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Contactin-1 in direct ELISAs. Detects human, mouse, and rat Contactin-1 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
CAA79696
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Anti-Neurofascin-155 IgG4 antibodies prevent paranodal complex formation in vivo.
Early Nodal and Paranodal Disruption in Autoimmune Optic Neuritis.
De novo fatty acid synthesis by Schwann cells is essential for peripheral nervous system myelination.
Antibodies against peripheral nerve antigens in chronic inflammatory demyelinating polyradiculoneuropathy.
Schwannomin-interacting Protein 1 Isoform IQCJ-SCHIP1 Is a Multipartner Ankyrin- and Spectrin-binding Protein Involved in the Organization of Nodes of Ranvier.
Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system.
Lipid raft-dependent endocytosis of close homolog of adhesion molecule L1 (CHL1) promotes neuritogenesis.
Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth.
Versican V2 assembles the extracellular matrix surrounding the nodes of ranvier in the CNS.
Knockdown of contactin-1 expression suppresses invasion and metastasis of lung adenocarcinoma.
Contactin associates with sodium channel Nav1.3 in native tissues and increases channel density at the cell surface.
Manso C, Querol L, Lleixà C, Poncelet M, Mekaouche M, Vallat JM, Illa I, Devaux JJ
The Journal of clinical investigation 2019 Mar 14;129(6):2222-2236
The Journal of clinical investigation 2019 Mar 14;129(6):2222-2236
Early Nodal and Paranodal Disruption in Autoimmune Optic Neuritis.
Stojic A, Bojcevski J, Williams SK, Diem R, Fairless R
Journal of neuropathology and experimental neurology 2018 May 1;77(5):361-373
Journal of neuropathology and experimental neurology 2018 May 1;77(5):361-373
De novo fatty acid synthesis by Schwann cells is essential for peripheral nervous system myelination.
Montani L, Pereira JA, Norrmén C, Pohl HBF, Tinelli E, Trötzmüller M, Figlia G, Dimas P, von Niederhäusern B, Schwager R, Jessberger S, Semenkovich CF, Köfeler HC, Suter U
The Journal of cell biology 2018 Apr 2;217(4):1353-1368
The Journal of cell biology 2018 Apr 2;217(4):1353-1368
Antibodies against peripheral nerve antigens in chronic inflammatory demyelinating polyradiculoneuropathy.
Querol L, Siles AM, Alba-Rovira R, Jáuregui A, Devaux J, Faivre-Sarrailh C, Araque J, Rojas-Garcia R, Diaz-Manera J, Cortés-Vicente E, Nogales-Gadea G, Navas-Madroñal M, Gallardo E, Illa I
Scientific reports 2017 Oct 31;7(1):14411
Scientific reports 2017 Oct 31;7(1):14411
Schwannomin-interacting Protein 1 Isoform IQCJ-SCHIP1 Is a Multipartner Ankyrin- and Spectrin-binding Protein Involved in the Organization of Nodes of Ranvier.
Martin PM, Cifuentes-Diaz C, Devaux J, Garcia M, Bureau J, Thomasseau S, Klingler E, Girault JA, Goutebroze L
The Journal of biological chemistry 2017 Feb 10;292(6):2441-2456
The Journal of biological chemistry 2017 Feb 10;292(6):2441-2456
Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system.
Çolakoğlu G, Bergstrom-Tyrberg U, Berglund EO, Ranscht B
Proceedings of the National Academy of Sciences of the United States of America 2014 Jan 21;111(3):E394-403
Proceedings of the National Academy of Sciences of the United States of America 2014 Jan 21;111(3):E394-403
Lipid raft-dependent endocytosis of close homolog of adhesion molecule L1 (CHL1) promotes neuritogenesis.
Tian N, Leshchyns'ka I, Welch JH, Diakowski W, Yang H, Schachner M, Sytnyk V
The Journal of biological chemistry 2012 Dec 28;287(53):44447-63
The Journal of biological chemistry 2012 Dec 28;287(53):44447-63
Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth.
Lieberoth A, Splittstoesser F, Katagihallimath N, Jakovcevski I, Loers G, Ranscht B, Karagogeos D, Schachner M, Kleene R
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 May 20;29(20):6677-90
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 May 20;29(20):6677-90
Versican V2 assembles the extracellular matrix surrounding the nodes of ranvier in the CNS.
Dours-Zimmermann MT, Maurer K, Rauch U, Stoffel W, Fässler R, Zimmermann DR
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Jun 17;29(24):7731-42
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Jun 17;29(24):7731-42
Knockdown of contactin-1 expression suppresses invasion and metastasis of lung adenocarcinoma.
Su JL, Yang CY, Shih JY, Wei LH, Hsieh CY, Jeng YM, Wang MY, Yang PC, Kuo ML
Cancer research 2006 Mar 1;66(5):2553-61
Cancer research 2006 Mar 1;66(5):2553-61
Contactin associates with sodium channel Nav1.3 in native tissues and increases channel density at the cell surface.
Shah BS, Rush AM, Liu S, Tyrrell L, Black JA, Dib-Hajj SD, Waxman SG
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Aug 18;24(33):7387-99
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Aug 18;24(33):7387-99
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Contactin-1 by Western Blot. Western blot shows lysates of human brain (cortex) tissue, human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/ Rat Contactin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Contactin-1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Contactin-1 by Simple WesternTM. Simple Western lane view shows lysates of human brain tissue and mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin-1 at approximately 158 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Contactin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Contactin-1 in Human Dorsal Root Ganglia. Contactin-1 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using 15 µg/mL Goat Anti-Human/ Mouse/Rat Contactin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by Contactin-1 and Neutralization by Human Contactin-1 Antibody. Recombinant Human Contactin-1 Fc Chimera (Catalog # 904-CN), immobilized onto a microplate, supports the adhesion of the C6 rat glioma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Contactin-1 Fc Chimera (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Contactin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904). The ND50 is typically 1-5 µg/mL.