NBP2-37757

antibody from Novus Biologicals

Targeting: ZCRB1 MADP-1, MADP1, RBM36, SNRNP31, ZCCHC19

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP2-37757

Provider

Novus Biologicals

Product name

Rabbit Polyclonal ZCRB1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ZCRB1 Antibody [NBP2-37757] - Detection of Human ZCRB1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZCRB1 antibody used for IP at 6 ug per reaction. ZCRB1 was also immunoprecipitated by rabbit anti-ZCRB1 antibody. For blotting immunoprecipitated ZCRB1, NBP2-37757 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ZCRB1 Antibody [NBP2-37757] - Detection of Human and Mouse ZCRB1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZCRB1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.