PA5-96542

antibody from Invitrogen Antibodies

Targeting: MANF ARMET, ARP

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96542

Provider

Invitrogen Antibodies

Product name

ARMET Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL Positive Samples: U-251MG, MCF7, PC-3

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.74 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ARMET was performed in U2OS cells using ARMET Polyclonal Antibody (Product # PA5-96542).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ARMET in HeLa cells. Samples were incubated with ARMET Polyclonal antibody (Product # PA5-96542) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ARMET in NIH/3T3 cells. Samples were incubated with ARMET Polyclonal antibody (Product # PA5-96542) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ARMET in PC-12 cells. Samples were incubated with ARMET Polyclonal antibody (Product # PA5-96542) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ARMET in U2OS cells. Samples were incubated with ARMET Polyclonal antibody (Product # PA5-96542) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of ARMET in 300 μg extracts of HepG2 cells. Samples were precipitated with 3 μg ARMET Polyclonal antibody (Product # PA5-96542). Western blot was performed from the immunoprecipitate using ARMET Polyclonal antibody (Product # PA5-96542) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ARMET in paraffin-embedded human tonsil. Samples were incubated with ARMET Polyclonal antibody (Product # PA5-96542) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ARMET in paraffin-embedded rat spleen. Samples were incubated with ARMET Polyclonal antibody (Product # PA5-96542) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ARMET in paraffin-embedded mouse spleen. Samples were incubated with ARMET Polyclonal antibody (Product # PA5-96542) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.