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- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- NBP1-76836 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-76836, RRID:AB_11003348
- Product name
- Rabbit Polyclonal MANF Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. This antibody does not cross-react with CDNF.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MANF Antibody [NBP1-76836] - Mouse brain tissue lysate with ARMET antibody at (A) 1 and (B) 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MANF Antibody [NBP1-76836] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76836 (Lane 1: 0.125 ug /mL, Lane 2: 0.25 ug/mL and Lane 3: 0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MANF Antibody [NBP1-76836] - Western Blot Validation in Human, Mouse (NIH/3T3) and Rat (YB2/0) Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: MANF NBP1-76836 (2 ug /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MANF Antibody [NBP1-76836] - KO Validation in HEK293T Cells. Loading: 10 ug of HEK293T WT cell lysates or MANF KO cell lysates. Antibodies: MANF NBP1-76836 (1 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MANF Antibody [NBP1-76836] - Analysis of MANF in human brain tissue with MANF antibody at 2.5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MANF Antibody [NBP1-76836] - Human brain tissue with ARMET antibody at 2.5 ug/ml.
