Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-76837 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-76837, RRID:AB_11019583
- Product name
- Rabbit Polyclonal MANF Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. This antibody does not cross-react with CDNF.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MANF Antibody [NBP1-76837] - Rat brain tissue lysate with ARMET antibody at (A) 1 and (B) 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76837 (Lane 1: 0.125 ug/mL, Lane 2: 0.25 ug/mL and Lane 3: 0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: MANF NBP1-76837, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MANF Antibody [NBP1-76837] - Human brain tissue with ARMET antibody at 2.5 ug/ml.