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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [1]
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- Product number
- MAB4028 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Myocardin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse Myocardin in Western blots.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8IZQ8- Isotype
- IgG
- Antibody clone number
- 355521
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Alpha-enolase regulates the malignant phenotype of pulmonary artery smooth muscle cells via the AMPK-Akt pathway.
Down-regulation of Insulin Receptor Substrate 1 during Hyperglycemia Induces Vascular Smooth Muscle Cell Dedifferentiation.
miR-17/20 Controls Prolyl Hydroxylase 2 (PHD2)/Hypoxia-Inducible Factor 1 (HIF1) to Regulate Pulmonary Artery Smooth Muscle Cell Proliferation.
Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity.
Co-activation of nuclear factor-κB and myocardin/serum response factor conveys the hypertrophy signal of high insulin levels in cardiac myoblasts.
Transplantation of mesenchymal cells rejuvenated by the overexpression of telomerase and myocardin promotes revascularization and tissue repair in a murine model of hindlimb ischemia.
Akt1 mediates α-smooth muscle actin expression and myofibroblast differentiation via myocardin and serum response factor.
Hypertension impairs myocardin function: a novel mechanism facilitating arterial remodelling.
Induction of intracellular heat-shock protein 72 prevents the development of vascular smooth muscle cell calcification.
down-regulation of Kruppel-like factor-4 (KLF4) by microRNA-143/145 is critical for modulation of vascular smooth muscle cell phenotype by transforming growth factor-beta and bone morphogenetic protein 4.
Dai J, Zhou Q, Chen J, Rexius-Hall ML, Rehman J, Zhou G
Nature communications 2018 Sep 21;9(1):3850
Nature communications 2018 Sep 21;9(1):3850
Down-regulation of Insulin Receptor Substrate 1 during Hyperglycemia Induces Vascular Smooth Muscle Cell Dedifferentiation.
Xi G, Wai C, White MF, Clemmons DR
The Journal of biological chemistry 2017 Feb 3;292(5):2009-2020
The Journal of biological chemistry 2017 Feb 3;292(5):2009-2020
miR-17/20 Controls Prolyl Hydroxylase 2 (PHD2)/Hypoxia-Inducible Factor 1 (HIF1) to Regulate Pulmonary Artery Smooth Muscle Cell Proliferation.
Chen T, Zhou Q, Tang H, Bozkanat M, Yuan JX, Raj JU, Zhou G
Journal of the American Heart Association 2016 Dec 5;5(12)
Journal of the American Heart Association 2016 Dec 5;5(12)
Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity.
Yao Y, Chen ZL, Norris EH, Strickland S
Nature communications 2014 Mar 3;5:3413
Nature communications 2014 Mar 3;5:3413
Co-activation of nuclear factor-κB and myocardin/serum response factor conveys the hypertrophy signal of high insulin levels in cardiac myoblasts.
Madonna R, Geng YJ, Bolli R, Rokosh G, Ferdinandy P, Patterson C, De Caterina R
The Journal of biological chemistry 2014 Jul 11;289(28):19585-98
The Journal of biological chemistry 2014 Jul 11;289(28):19585-98
Transplantation of mesenchymal cells rejuvenated by the overexpression of telomerase and myocardin promotes revascularization and tissue repair in a murine model of hindlimb ischemia.
Madonna R, Taylor DA, Geng YJ, De Caterina R, Shelat H, Perin EC, Willerson JT
Circulation research 2013 Sep 13;113(7):902-14
Circulation research 2013 Sep 13;113(7):902-14
Akt1 mediates α-smooth muscle actin expression and myofibroblast differentiation via myocardin and serum response factor.
Abdalla M, Goc A, Segar L, Somanath PR
The Journal of biological chemistry 2013 Nov 15;288(46):33483-93
The Journal of biological chemistry 2013 Nov 15;288(46):33483-93
Hypertension impairs myocardin function: a novel mechanism facilitating arterial remodelling.
Pfisterer L, Feldner A, Hecker M, Korff T
Cardiovascular research 2012 Oct 1;96(1):120-9
Cardiovascular research 2012 Oct 1;96(1):120-9
Induction of intracellular heat-shock protein 72 prevents the development of vascular smooth muscle cell calcification.
Lu TS, Lim K, Molostvov G, Yang YC, Yiao SY, Zehnder D, Hsiao LL
Cardiovascular research 2012 Dec 1;96(3):524-32
Cardiovascular research 2012 Dec 1;96(3):524-32
down-regulation of Kruppel-like factor-4 (KLF4) by microRNA-143/145 is critical for modulation of vascular smooth muscle cell phenotype by transforming growth factor-beta and bone morphogenetic protein 4.
Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G, Hata A
The Journal of biological chemistry 2011 Aug 12;286(32):28097-110
The Journal of biological chemistry 2011 Aug 12;286(32):28097-110
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Myocardin by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
