PA5-13571

antibody from Invitrogen Antibodies

Targeting: SRSF5 HRS, SFRS5, SRP40

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-13571

Provider

Invitrogen Antibodies

Product name

SRSF5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse and rat based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

1.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

MG132-induced progerin clearance is mediated by autophagy activation and splicing regulation.

Harhouri K, Navarro C, Depetris D, Mattei MG, Nissan X, Cau P, De Sandre-Giovannoli A, Lévy N
EMBO molecular medicine 2017 Sep;9(9):1294-1313

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of NCI-H292 cells using a SFRS5 polyclonal antibody (Product # PA5-13571) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 MG 132 reduces progerin transcripts and splicing factor SRSF -1 protein expression, while SRSF -5 protein levels are increased Upper panel, lamin A/C, progerin, and GAPDH expressions in whole lysates from HGPS fibroblasts treated for 48 h either with DMSO control (-) or with (+) the indicated drug (s) (MG132 (5 muM), chloroquine (50 muM), bafilomycin A1 (Baf. A1) (100 nM), caspase-6 inhibitor (Casp-6 inh.) (50 muM) or leptomycin B (Lept. B) (20 ng/ml). Lower panels, progerin expression levels in drug (s)-treated cells relative to DMSO-treated cells (well no. 1) were normalized to GAPDH values. ( n = 5). Downregulation of progerin transcripts in response to MG132. Quantitative real-time PCR analyses of progerin mRNA levels in HGPS fibroblasts treated with 5 muM MG132 relative to DMSO-treated cells (Control: ""C""). ( n = 3). A representative Western blotting experiment in whole lysates of HGPS fibroblasts showing progerin, lamin A, lamin C, actin, GAPDH and SRSF-1 expression in MG132-treated HGPS cells up to 96 h and relative to DMSO-treated cells ""C"". Urea was used to lyse cells. ( n = 4). Proteasome activities in HGPS fibroblasts treated with 5 muM MG132 relative to DMSO-treated cells, (Control: untreated cells). ( n = 3). MG132-mediated SRSF-5 accumulation and SRSF-1 downregulation correlate with progerin clearance. A representative Western blotting experiment in whole lysates of HGPS fibroblasts showing progerin, SRSF-5, GAPDH, SRSF-1, LC3BI, and LC3BII expressi