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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [6]
- Immunohistochemistry [4]
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- Product number
- R31751 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- LYRIC Antibody / Metadherin / AEG-1
- Antibody type
- Polyclonal
- Description
- This highly specific LYRIC antibody is suitable for use in Western blot/Immunohistochemistry/Immunocytochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the LYRIC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of LYRIC antibody and Lane 1: rat skeletal muscle; 2: mouse skeletal muscle; 3: (r) heart tissue; 4: (m) heart; 5: human MCF-7; 6: (h) U87; 7: (h) 22RV1; 8: (r) PC12; Predicted size: 64-75KD; Observed size: 100KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) U-87 MG, 2) HeLa, 3) MDA-MB-453, 4) PC-3, 5) A431, 6) Caco-2 and 7) HepG2 cell lysate with LYRIC antibody. Expected molecular weight: 70-80 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human SMMC-7721 cells with LYRIC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human A549 cells with LYRIC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human HeLa cells with LYRIC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human SMMC-7721 cells with LYRIC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human A549 cells with LYRIC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human HeLa cells with LYRIC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: LYRIC antibody testing of human breast cancer tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: LYRIC antibody testing of rat skeletal muscle tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: LYRIC antibody testing of human breast cancer tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: LYRIC antibody testing of rat skeletal muscle tissue
