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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab113812 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant LYRIC/AEG1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human LYRIC/AEG1 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.Application:WB: 1/500 - 1/1000IHC: 1/50-1/100IF/ICC: 1/50 - 1/200Protein FunctionDownregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 214-440- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,6 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant LYRIC/AEG1 Antibody ( Ab113812 ) - Western Blot All lanes: Recombinant LYRIC/AEG1 Antibody (Ab113812) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 70, 76 kDa Exposure time: 50.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant LYRIC/AEG1 Antibody (Ab113812) - IF/ICC Immunocytochemistry analysis of LYRIC (green) in HeLa using Recombinant LYRIC/AEG1 Antibody (Ab113812), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant LYRIC/AEG1 Antibody (Ab113812) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant LYRIC/AEG1 Antibody (Ab113812). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
