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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ7375 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- AEG-1 Antibody / Astrocyte elevated gene-1 / MTDH / LYRIC
- Antibody type
- Monoclonal
- Description
- This highly specific AEG-1 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 2E5G3
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the AEG-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) U-87 MG, 2) HeLa, 3) PC-3 and 4) HepG2 cell lysate with AEG-1 antibody. Expected molecular weight: 70-80 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colorectal adenocarcinoma tissue with AEG-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with AEG-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AEG-1 antibody.
