Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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Comment
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- Product number
- H00010486-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010486-M01, RRID:AB_425850
- Product name
- CAP2 monoclonal antibody (M01), clone 3G9-1A5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant CAP2.
- Antigen sequence
MANMQGLVERLERAVSRLESLSAESHRPPGNCGEV
NGVIAGVAPSVEAFDKLMDSMVAEFLKNSRILAGD
VETHAEMVHSAFQAQRAFLLMASQYQQPHENDVAA
LLKPISEKIQEIQTFRERNRGSNMFNHLSAVSESI
PALGWIAVSPKPGPYVKEMNDAATFYTNRVLKDYK
HSDLRHVDWVKSYLNIWSELQAYIKEHHTTGLTWS
KTGPVASTVSAFSVLSSGPGLPPPPPPLPPPGPPP
LFENEGKKEESSPSRSALFAQLNQGEAITKGLRHV
TDDQKTYKNPSLRAQGGQTQSPTKSHTPSPTSPKS
YPSQKHAPVLELEGKKWRVEYQEDRNDLVISETEL
KQVAYIFKCEKSTIQIKGKVNSIIIDNCKKLGLVF
DNVVGIVEVINSQDIQIQVMGRVPTISINKTEGCH
IYLSEDALDCEIVSAKSSEMNILIPQDGDYREFPI
PEQFKTAWDGSKLITEPAEIMA- Isotype
- IgG
- Antibody clone number
- 3G9-1A5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CAP2 monoclonal antibody (M01), clone 3G9-1A5 Western Blot analysis of CAP2 expression in C32 ( Cat # L002V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CAP2 is approximately 10ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to CAP2 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol