LS-C396856

antibody from LSBio

Targeting: NDUFB6 B17, CI

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C396856

Provider

LSBio

Product name

NDUFB6 Antibody LS-C396856

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Positive WB detected in: HEK293 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse spleen tissue, Mouse lung tissue, Mouse kidney tissue, Mouse brain tissue, Mouse skeletal muscle tissue, Mouse thymus tissue All lanes: NDUFB6 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16, 14 kDa Observed band size: 16, 14 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating NDUFB6 in HEK293 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of NDUFB6 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: NDUFB6 Antibody (4µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating NDUFB6 in HEK293 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of NDUFB6 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: NDUFB6 Antibody (4µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg)