HPA045128

antibody from Atlas Antibodies

Targeting: RPP38

Immunocytochemistry

Antibody data

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Validation data

Product number: HPA045128 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA045128, RRID:AB_2797230
Product name: Anti-RPP38
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human RPP38, Gene description: ribonuclease P/MRP 38kDa subunit, Validated applications: WB, ICC, Uniprot ID: P78345, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.2 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

RPP38 protein structure - HPA045128 shown in red.

Enhanced validation

Submitted by: klas2
Enhanced method: Genetic validation
Main image
Experimental details: Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting RPP38 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting RPP38 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
Sample type: U-2 OS
Primary Ab dilution: 1:161
Conjugate: Horseradish Peroxidase
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:3000
Knockdown/Genetic Approaches Application: Western blot
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Prod no: s20708
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Prod no 2: s20707
KD Reagent Page 3: KD Reagent Page 3
KD Reagent Prod no 3: s223132
Antibody Lot Number: R42254
Appendix: 566c635aba2e6.pdf

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RPP38 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Sample type: Human
Protocol: Protocol

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RPP38 antibody. Remaining relative intensity is presented
Sample type: Human
Protocol: Protocol