Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-30079 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LDHC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1. Predicted reactivity: Dog (89%), Pig (88%), Bovine (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Sperm proteins and cancer-testis antigens are released by the seminiferous tubules in mice and men.
Human prostasomes from normozoospermic and non-normozoospermic men show a differential protein expression pattern.
O'Donnell L, Rebourcet D, Dagley LF, Sgaier R, Infusini G, O'Shaughnessy PJ, Chalmel F, Fietz D, Weidner W, Legrand JMD, Hobbs RM, McLachlan RI, Webb AI, Pilatz A, Diemer T, Smith LB, Stanton PG
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Mar;35(3):e21397
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Mar;35(3):e21397
Human prostasomes from normozoospermic and non-normozoospermic men show a differential protein expression pattern.
García-Rodríguez A, de la Casa M, Peinado H, Gosálvez J, Roy R
Andrology 2018 Jul;6(4):585-596
Andrology 2018 Jul;6(4):585-596
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LDHC Polyclonal Antibody detects lactate dehydrogenase C protein at cytoplasm by immunofluorescent analysis. Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: lactate dehydrogenase C protein stained by LDHC Polyclonal Antibody (Product # PA5-30079) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- lactate dehydrogenase C antibody detects LDHC protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded ovarian carcinoma. Lactate dehydrogenase C antibody (Product # PA5-30079) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 4 Western blot analysis of a sperm-specific protein and CTA in TIF from men with normal vs abnormal spermatogenesis. Western blotting for LDHC was performed on 50 ug of TIF protein collected from n = 8 men with normal spermatogenesis based on testis biopsy (Obstructive Azoospermia, OA) and n = 8 men with a phenotype of presumed Sertoli cell-only (SCO), based on a spermatogenic score of 0; these men were unable to have sperm retrieved during microsurgical-assisted testicular sperm extraction (see Methods). Blots were scanned and statistical differences in band intensity between groups were calculated by the Mann-Whitney test. The experiment was repeated three times and a statistically significant difference with a P value of .03 was observed between OA and SCO samples in all three experiments. A representative experiment is shown here with the box plot indicating median, 1st and 3rd quartiles, and data points from individual patients
