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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-38037 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRBN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A recommended positive control is human testis tissue lysate.
- Concentration
- 1 mg/mL
Submitted references A Chemical Toolbox for Labeling and Degrading Engineered Cas Proteins.
Gama-Brambila RA, Chen J, Dabiri Y, Tascher G, Němec V, Münch C, Song G, Knapp S, Cheng X
JACS Au 2021 Jun 28;1(6):777-785
JACS Au 2021 Jun 28;1(6):777-785
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CRBN in human testis tissue lysate using a CRBN polyclonal antibody (Product # PA5-38037) at a dilution of (A) 0.5 µg/mL and (B) 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CRBN in human testis tissue lysate with CRBN Polyclonal Antibody (Product # PA5-38037) at (A) 0.5 and (B) 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Protein cereblon was achieved by transfecting HeLa with Protein cereblon specific siRNAs (Silencer® select Product # S13531, S27635). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Protein cereblon knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CRBN Polyclonal Antibody (Product # PA5-38037, 1:1000 ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Protein cereblon.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CRBN Polyclonal Antibody (Product # PA5-38037) and a 51 kDa band corresponding to Protein cereblon was observed across cell lines and tissues. Whole cell extracts (30 µg lysate) of PC-3 (Lane 1), THP-1 (Lane 2), HeLa (Lane 3), Mouse Brain (Lane 4), Rat Brain (Lane 5), Mouse Testis (Lane 6), Mouse Eye (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Protein cereblon was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CRBN Polyclonal Antibody (Product # PA5-38037) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of CRBN in rat testis tissue with CRBN Polyclonal Antibody (Product # PA5-38037) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of CRBN in rat testis tissue with CRBN Polyclonal Antibody (Product # PA5-38037) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of CRBN in rat testis tissue with CRBN Polyclonal Antibody (Product # PA5-38037) at 5 µg/mL.
