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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-30019 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Stomatin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116. Predicted reactivity: Human (99%), Mouse (96%), Rat (97%), Zebrafish (92%), Xenopus laevis (92%), Dog (98%), Rhesus Monkey (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Proteomics study of human cord blood reticulocyte-derived exosomes.
Díaz-Varela M, de Menezes-Neto A, Perez-Zsolt D, Gámez-Valero A, Seguí-Barber J, Izquierdo-Useros N, Martinez-Picado J, Fernández-Becerra C, Del Portillo HA
Scientific reports 2018 Sep 19;8(1):14046
Scientific reports 2018 Sep 19;8(1):14046
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 3 Proteome of human reticulocyte-derived exosomes identified by LC-MS/MS. ( A ) Western blot validation for HSP70, GAPDH and stomatin on UC- HuRex . Samples were purified from two cord blood donors, each one loaded in a different lane. (B) Distribution of the proteome of HuRex in subcellular location categories retrieved from UniProt database according to Gene Ontology (GO) annotation. (C) Venn diagram showing the overlap of proteins detected in HuRex and those reported in Vesiclepedia, a database of extracellular vesicle cargo 30 . (D) GO term-enrichment analysis of HuRex proteome at cellular component and molecular function level performed with Database for Annotation, Visualization and Integrated Discovery (David 6.8) 60 . The most over-represented GO terms are shown.
