22658-1-AP

antibody from Invitrogen Antibodies

Targeting: EYA1 BOR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

22658-1-AP

Provider

Invitrogen Antibodies

Product name

EYA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MEMQDLTSP HSRLSGSSES PSGPKLGNSH INSNSMTPNG TEVKTEPMSS SETASTTADG SLNNFSGSAI GSSSFSPRPT HQFSPPQIYP SNRPYPHILP TPSSQTMAAY GQTQFTTGMQ QATAYATYPQ PGQPYGISSY GALWAGIKTE GGLSQSQSPG QTGFLSYGTS FSTPQPGQAP YSYQMQGSSF TTSSGIYTGN NSLTNSSGFN SSQQDYPSYP SFGQGQYAQY YNSSPYPAHY MTSSNTSPTT PSTNATYQLQ EPPSGITSQA VTDPTAEYST IHSPSTPIKD SDSDRLRRGS D (1-300 aa encoded by BC121799)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse colon tissue were subjected to SDS PAGE followed by western blot with 22658-1-AP (EYA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 22658-1-AP (EYA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22658-1-AP ( EYA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NCCIT cell were subjected to SDS PAGE followed by western blot with 22658-1-AP (EYA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 22658-1-AP (EYA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 22658-1-AP (EYA1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 22658-1-AP (EYA1 antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EYA1 (IP:22658-1-AP, 4ug; Detection:22658-1-AP 1:300) with mouse kidney tissue lysate 5300ug.