Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN954395 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Pygopus Homolog 1 (Drosophila) (PYGO1) (C-Term), (AA 389-417) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 389-417 amino acids from the C-terminal region of Human PYGO1
- Description
- Affinity Chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 389-417
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Pygopus residues required for its binding to Legless are critical for transcription and development.
Wnt/wingless signaling requires BCL9/legless-mediated recruitment of pygopus to the nuclear beta-catenin-TCF complex.
Townsley FM, Thompson B, Bienz M
The Journal of biological chemistry 2004 Feb 13;279(7):5177-83
The Journal of biological chemistry 2004 Feb 13;279(7):5177-83
Wnt/wingless signaling requires BCL9/legless-mediated recruitment of pygopus to the nuclear beta-catenin-TCF complex.
Kramps T, Peter O, Brunner E, Nellen D, Froesch B, Chatterjee S, Murone M, Züllig S, Basler K
Cell 2002 Apr 5;109(1):47-60
Cell 2002 Apr 5;109(1):47-60
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of PYGO1 Antibody (C-term) Cat.-No AP53530PU-N in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the PYGO1 antibody detected the PYGO1 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PYGO1 Antibody (C-term) Cat.-No AP53530PU-N with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human placenta tissue reacted with PYGO1 Antibody (C-term) Cat.-No AP53530PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.