PA5-77257

antibody from Invitrogen Antibodies

Targeting: COL5A3

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-77257

Provider

Invitrogen Antibodies

Product name

COL5A3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Global targetome analysis reveals critical role of miR-29a in pancreatic stellate cell mediated regulation of PDAC tumor microenvironment.

Dey S, Liu S, Factora TD, Taleb S, Riverahernandez P, Udari L, Zhong X, Wan J, Kota J
BMC cancer 2020 Jul 13;20(1):651

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 Validation of miR-29a direct target. a Relative fold changes estimated by qPCR analysis for the top miR-29a candidate target genes of ITGA6, ADAMTS2, IGF-1, COL5A3, CLDN1, E2F7 and MYBL2 in hPSCs transfected with miR-29a mimics (29a OE) compared with cells transfected with scramble control (CTRL). Numerical data are represented as average fold change (DeltaDeltaCT) +- standard error of the mean (SEM); ** p < 0.01; n = 3. b Total protein harvested from the hPSCs transfected with scramble control (CTRL) or miR-29a mimics (29a OE) 48 h post-transfection were subjected to western blot analysis for miR-29a candidate targets of ITGA6, ADAMTS2, IGF-1, COL5A3, CLDN1, E2F7 and MYBL2. GAPDH was used as the loading control. Quantification of band intensities normalized to GAPDH. Quantification of band intensities normalized to GAPDH and relative to respective controls are represented as +- SEM; n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001 (right). Uncropped blots are shown in Additional file 3 : Fig. S1