PA5-30874

antibody from Invitrogen Antibodies

Targeting: KIF13A bA500C11.2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30874

Provider

Invitrogen Antibodies

Product name

KIF13A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, HepG2. Predicted reactivity: Mouse (93%), Rat (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation.

Birdsall V, Kirwan K, Zhu M, Imoto Y, Wilson SM, Watanabe S, Waites CL
Life science alliance 2022 Oct;5(10)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KIF13A in methanol-fixed HeLa cells using a KIF13A polyclonal antibody (Product # PA5-30874) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure S8. Knockdown of KIF13A and KIF13B in neurons. (A) Immunoblot of lysates from 13 to 15 day in vitro (DIV) hippocampal neurons expressing shKIF13A1, shKIF13A2, or scrambled shRNA control (shCtrl), probed with antibodies against KIF13A and tubulin. (B) Quantification of KIF13A band intensity, normalized to tubulin control, with shCtrl or shKIF13A expression (*** P = 0.0004 [shKIF13A1], *** P = 0.0002 [shKIF13A2], one-way ANOVA with Dunnett's multiple comparisons test; n = 4 independent experiments). Bars show mean +- SEM. (C) Images of axons from 14 DIV dissociated hippocampal neurons expressing shCtrl or shKIF13A1, immunostained for neurofilament and KIF13A. (D) Quantification of KIF13A puncta per 100 mum axon (** P = 0.0012, unpaired t test, n = 18 [shCtrl] and 15 [shKIF13A1] fields of view/condition). (E) Representative immunoblot of lysates from 13 to 15 DIV hippocampal neurons expressing shKIF13B or scrambled shRNA control (shCtrl), probed with antibodies against KIF13B and tubulin. (F) Quantification of KIF13B band intensity, normalized to tubulin control, with shCtrl or shKIF13B expression (*** P = 0.0007, unpaired t test; n = 4 independent experiments). Bars show mean +- SEM. (G) Images and corresponding kymographs of EGFP-Hrs in axons from dissociated hippocampal neurons expressing shCtrl (left panels), shKIF13A2 (middle panels), or shKIF13B (right panels), under control conditions (upper panels) and after 2 h treatment with Bic/4AP (lower panels). Horizontal si