LS-C291001

antibody from LSBio

Targeting: CD226 DNAM-1, DNAM1, PTA1, TLiSA1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C291001

Provider

LSBio

Product name

Anti-DNAM-1 / CD226 Antibody (aa286-336) LS-C291001

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Horse

Host

Rabbit

Vial size

100µl

Storage

Store at 2-8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human DNAM-1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-DNAM-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human DNAM-1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-DNAM-1 antibody used for IP at 6 ug per reaction. DNAM-1 was also immunoprecipitated by rabbit anti-DNAM-1 antibody BL16224. For blotting immunoprecipitated DNAM-1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.