Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB4436 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal DNAM-1/CD226 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects mouse DNAM-1/CD226 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DNAM-1/CD226 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of DNAM-1/CD226 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD8 alpha APC-conjugated Monoclonal Antibody (Catalog # FAB116A) and either (A) Rat Anti-Mouse DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB4436) or (B) Rat IgG2B Isotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).