Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
Reference
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- Product number
- MAB666-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DNAM-1/CD226 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human DNAM-1/CD226 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q15762
- Isotype
- IgG
- Antibody clone number
- 102511
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors.
The Repeated Administration of Resveratrol Has Measurable Effects on Circulating T-Cell Subsets in Humans.
CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells.
Ascorbic acid promotes proliferation of natural killer cell populations in culture systems applicable for natural killer cell therapy.
Killing of myeloid APCs via HLA class I, CD2 and CD226 defines a novel mechanism of suppression by human Tr1 cells.
López-Cobo S, Pieper N, Campos-Silva C, García-Cuesta EM, Reyburn HT, Paschen A, Valés-Gómez M
Oncoimmunology 2018;7(2):e1392426
Oncoimmunology 2018;7(2):e1392426
The Repeated Administration of Resveratrol Has Measurable Effects on Circulating T-Cell Subsets in Humans.
Espinoza JL, Trung LQ, Inaoka PT, Yamada K, An DT, Mizuno S, Nakao S, Takami A
Oxidative medicine and cellular longevity 2017;2017:6781872
Oxidative medicine and cellular longevity 2017;2017:6781872
CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells.
Davis ZB, Sowrirajan B, Cogswell A, Ward JP, Planelles V, Barker E
AIDS research and human retroviruses 2017 Feb;33(2):93-100
AIDS research and human retroviruses 2017 Feb;33(2):93-100
Ascorbic acid promotes proliferation of natural killer cell populations in culture systems applicable for natural killer cell therapy.
Huijskens MJ, Walczak M, Sarkar S, Atrafi F, Senden-Gijsbers BL, Tilanus MG, Bos GM, Wieten L, Germeraad WT
Cytotherapy 2015 May;17(5):613-20
Cytotherapy 2015 May;17(5):613-20
Killing of myeloid APCs via HLA class I, CD2 and CD226 defines a novel mechanism of suppression by human Tr1 cells.
Magnani CF, Alberigo G, Bacchetta R, Serafini G, Andreani M, Roncarolo MG, Gregori S
European journal of immunology 2011 Jun;41(6):1652-62
European journal of immunology 2011 Jun;41(6):1652-62
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of DNAM-1/CD226 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by DNAM-1/CD226 and Neutral-ization by Human DNAM-1/CD226 Antibody. Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN), immobilized onto a microplate, supports the adhesion of the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human DNAM-1/CD226 Fc Chimera (20 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666). The ND50 is typically 0.1-0.6 µg/mL.