A700-063-T

antibody from Invitrogen Antibodies

Targeting: CD226 DNAM-1, DNAM1, PTA1, TLiSA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-063-T

Provider

Invitrogen Antibodies

Product name

DNAM-1/CD226 Recombinant Rabbit Monoclonal Antibody (BLR063G)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR063G

Vial size

10 µL

Concentration

1000 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DNAM-1/CD226 by western blot. Samples: Whole cell lysate (10 µg) from SUP-M2, RT-4, HH, U-251MG, MJ, HDLM-2, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-DNAM-1/CD226 recombinant monoclonal antibody [BL-1060-1D2] (Product # A700-063 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody (Product # A304-523A).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DNAM-1 in FFPE HH cells by ICC. Antibody: Rabbit anti-DNAM-1 recombinant monoclonal antibody [BLR063G] (Product # A700-063; lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DNAM-1 in FFPE Jurkat cells by ICC. Antibody: Rabbit anti-DNAM-1 recombinant monoclonal antibody [BLR063G] (Product # A700-063 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DNAM-1 in FFPE colon carcinoma by IHC. Antibody: Rabbit anti-DNAM-1 recombinant monoclonal antibody [BLR063G] (Product # A700-063 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DNAM-1/CD226 by immunohistochemistry. AntibodySample: FFPE section of lung carcinoma.Antibody: Rabbit anti-DNAM-1/CD226 recombinant monoclonal antibody (BLR063G) (A700-063 lot 1) used at 1:200.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DNAM-1/CD226 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HH cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-DNAM-1/CD226 recombinant monoclonal antibody [BL-1060-1D2] (Product # A700-063) used for IP at 20 µL/mg lysate. DNAM-1/CD226 was also immunoprecipitated by a second antibody against a different epitope of DNAM-1/CD226 (BL-799B-1B9). For blotting immunoprecipitated DNAM-1/CD226 (Product # A700-063) was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.