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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ6841 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- DNAM-1 Antibody / CD226
- Antibody type
- Polyclonal
- Description
- This highly specific DNAM-1 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the DNAM-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human Jurkat, 2) human HT1080, 3) human A549, 4) human HEL and 5) mouse thymus tissue lysate with DNAM-1 antibody. Expected molecular weight: 35-70 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human Jurkat cells with DNAM-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNAM-1 antibody.
