Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP32693PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti TGOLN2 / TGN46
- Antibody type
- Polyclonal
- Antigen
- Antisera to TGN46 were raised by repeated immunisations of Sheep with highly purified recombinant Human TGN46
- Reactivity
- Human, Simian
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.1 mg/ml
Submitted references Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network.
A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells.
Regulation of activity and localization of the WNK1 protein kinase by hyperosmotic stress.
Huntingtin interacting protein 1 modulates the transcriptional activity of nuclear hormone receptors.
HIV-1 Nef down-regulates the hemochromatosis protein HFE, manipulating cellular iron homeostasis.
A cycling cis-Golgi protein mediates endosome-to-Golgi traffic.
von Blume J, Duran JM, Forlanelli E, Alleaume AM, Egorov M, Polishchuk R, Molina H, Malhotra V
The Journal of cell biology 2009 Dec 28;187(7):1055-69
The Journal of cell biology 2009 Dec 28;187(7):1055-69
A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells.
Ganley IG, Espinosa E, Pfeffer SR
The Journal of cell biology 2008 Jan 14;180(1):159-72
The Journal of cell biology 2008 Jan 14;180(1):159-72
Regulation of activity and localization of the WNK1 protein kinase by hyperosmotic stress.
Zagórska A, Pozo-Guisado E, Boudeau J, Vitari AC, Rafiqi FH, Thastrup J, Deak M, Campbell DG, Morrice NA, Prescott AR, Alessi DR
The Journal of cell biology 2007 Jan 1;176(1):89-100
The Journal of cell biology 2007 Jan 1;176(1):89-100
Huntingtin interacting protein 1 modulates the transcriptional activity of nuclear hormone receptors.
Mills IG, Gaughan L, Robson C, Ross T, McCracken S, Kelly J, Neal DE
The Journal of cell biology 2005 Jul 18;170(2):191-200
The Journal of cell biology 2005 Jul 18;170(2):191-200
HIV-1 Nef down-regulates the hemochromatosis protein HFE, manipulating cellular iron homeostasis.
Drakesmith H, Chen N, Ledermann H, Screaton G, Townsend A, Xu XN
Proceedings of the National Academy of Sciences of the United States of America 2005 Aug 2;102(31):11017-22
Proceedings of the National Academy of Sciences of the United States of America 2005 Aug 2;102(31):11017-22
A cycling cis-Golgi protein mediates endosome-to-Golgi traffic.
Natarajan R, Linstedt AD
Molecular biology of the cell 2004 Nov;15(11):4798-806
Molecular biology of the cell 2004 Nov;15(11):4798-806
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- 20μg HeLa cell lysate was run on an SDS-PAGE gel and western blotted using Affinity purified Sheep anti-Human TGN46 Antibody Cat.-No AP32693PU-Nat 1/1000 dilution.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Human TGN46 in 3% paraformaldehyde-fixed HeLa cells using Affinity purified Sheep anti-Human TGN46 Antibody Cat.-No AP32693PU-NÂ at 1/100. Secondary antibody was Donkey anti-Sheep Alexa Fluor 488.