PA1-1069

antibody from Invitrogen Antibodies

Targeting: TGOLN2 TGN38, TGN46, TGN48, TGN51, TTGN2

Antibody data

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Validation data

Product number: PA1-1069 - Provider product page
Provider: Invitrogen Antibodies
Product name: TGN46 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA1-1069 detects TGN46 in human samples. PA1-1069 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~51 kDa protein representing TGN46 in HEK293 and HeLa cell lysates. The PA1-1069 immunogen is a synthetic peptide corresponding to residues V(252) V P E Q P S W K D H S K P I S(267) of human TGN46. This peptide (Cat. # PEP-291) is available for use in neutralization and control experiments.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

TGOLN2 protein structure - PA1-1069 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using Anti-TGN46 Polyclonal Antibody (Product # PA1-1069) and an 80-110 kDa band corresponding to Tgoln2 was observed across all the cell lines tested. A non-specific band (*) was seen above 15 kDa. Whole-cell extracts (30 µg lysate) of T98G (Lane 1), and Hep G2 (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Tgoln2 was achieved by transfecting T98G with Tgoln2-specific siRNAs (Silencer® select Product # S20855, S20854). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Tgoln2 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2), and untransfected cells (lane 1). The blot was probed with TGN46 Polyclonal Antibody (Product # PA1-1069, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution). Densitometric analysis of this western blot is shown in the histogram (Fig. b). A decrease in signal upon siRNA-mediated knockdown confirms that the antibody is specific to Tgoln2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Trans Golgi Network 46 using anti-Trans Golgi Network 46 polyclonal antibody (Product # PA1-1069) shows staining in HMVEC Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Trans Golgi Network 46 using anti-Trans Golgi Network 46 polyclonal antibody (Product # PA1-1069) shows staining in p19 Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Trans-Golgi Network 46 was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Trans-Golgi Network 46 Rabbit Polyclonal Antibody (Product # PA1-1069) at 2µg/mLin 0.1% BSA, incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Trans Golgi Network 46 using anti-Trans Golgi Network 46 polyclonal antibody (Product # PA1-1069) shows staining in A549 Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Trans Golgi Network 46 using anti-Trans Golgi Network 46 polyclonal antibody (Product # PA1-1069) shows staining in HMVEC Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of TGN46 in MCF7 cells. MCF7 cells ectopically expressing GALT1-eGFP were stained with a TGN46 polyclonal antibody (Product # PA1-1069) at a dilution of 1:100, with an incubation overnight at 4C, washed extensively followed by an incubation with anti-rabbit secondary antibody at RT for one hour. Confocal imaging was performed at 60X magnification. Green: eGFP tagged GALT1; Red: TGN46 staining. Data courtesy of Dr Wei Xu's lab at University of Wisconsin Madison.