MA3-063

antibody from Invitrogen Antibodies

Targeting: TGOLN2 TGN38, TGN46, TGN48, TGN51, TTGN2

Provider product page for MA3-063

Western blot

Immunocytochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [55]
Comments [0]
Validations
Immunocytochemistry [4]
Other assay [6]

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Product number: MA3-063 - Provider product page
Provider: Invitrogen Antibodies
Product name: TGN46 Monoclonal Antibody (2F7.1)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: MA3-063 detects trans-Golgi network (TGN) 38 from human and monkey samples and both recombinant and endogenous rat samples. MA3-063 has been successfully used in Western blot, immunofluorescence, immunocytochemistry and FACS procedures. By Western blot, this antibody detects an ~38 kDa protein representing deglycosylated recombinant rat TGN38. Under normal Western blot conditions, this antibody detects an 85-95 kDa band depending on cell type. Immunofluorescence staining of TGN38 in NRK cells with MA3-063 results in staining predominantly in the TGN. In immunofluorescence, it has been shown that methanol works best as a fixative. The MA3-063 immunogen is a synthetic peptide corresponding to residues L(18) P S A S K P N N T S S E N N P P(34) C of rat TGN38.
Reactivity: Human, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 2F7.1
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

TGOLN2 protein structure - MA3-063 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: HeLa and COS7 cells were rinsed with PBS and fixed with -20°C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody (Product # MA3-063) diluted 1:100 in PBS containing 0.2%BSA for 1 hour. The cells were then rinsed in PBS and incubated with goat anti-mouse IgG conjugated to rhodamine for 30 minutes in PBS containing 0.2% BSA. Following washing, cells were imaged using a Zeiss Axioplan microscope. Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Trans Golgi Network 38 using anti-Trans Golgi Network 38 monoclonal antibody (Product # MA3-063) shows staining in NS-1 Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a TGN38 monoclonal antibody (Product # MA3-063) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 680 goat anti-mouse IgG secondary antibody (Product # 35518) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/mL final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NRK cells were stained with anti-TGN38 (red, MA3-063), anti-beta-COP (green, Product # PA1-061), and DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 6 ATP7A and ATP7B co-localize differentially with the TGN markers TGN38 and Golgin-97. Representative confocal images of proliferating and differentiating myoblasts immunostained for: (A) TGN38 (green), ATP7A (cyan), ATP7b (red) and DAPI (blue). (B) Golgin-97 (green), ATP7A (cyan), ATP7b (red) and DAPI (blue). Images depicted are representative of three independent biological experiments.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7 Involvement of the Golgi apparatus in leptin transcytosis . Caco-2 cells were exposed to leptin-FITC for 30 minutes. (a) By confocal microscopy, leptin (green fluorescence) and TGN42, a marker of the Golgi apparatus (red fluorescence), appear colocalized (yellow staining) in spots ( X Z plane) or as crescent-shaped structures close to the nuclei ( X Y plane). (b) Quantification of signal for leptin and TGN42 in X Z view confirmed colocalization. (c) Inhibitors of Golgi trafficking, brefeldin A (Bref) (10 mu g/mL), and okadaic acid (Okad.a) (0.5 mu M) were added to the cell medium for 30 minutes prior adding leptin. Cellular content (c1) and concentrations of leptin in the basal medium (c2) were decreased by the two agents. Results are expressed as mean values +- s.e.m ( n = 4).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown p110delta attenuated TNFalpha and IL-6 secretions by disturbing their trafficking out of Golgi complex in TiPs-stimulated macrophages. A TiPs-induced secretions of TNFalpha and IL-6 from p110delta-knockdown macrophages were detected by ELISA assay. B mRNA productions of TNFalpha and IL-6 were examined by qPCR assay in p110delta-knockdown RAW264.7 upon 8 h TiPs stimulation. C Protein levels of TNFalpha and IL-6 were analysed by WB assay after 8 h TiPs stimulation with Brefeldin A (BFA), an inhibitor to hinder the transit from Endoplasmic Reticulum (ER) to Golgi complex. D , E After treated with TiPs for 2 h, p110delta-knockdown RAW264.7 were imaged with confocal microscopy to detect TNFalpha distribution. D Macrophages were co-treated with TiPs and 10 muM TAPI-1 to detect the surface distribution without permeabilization. It reflected the secretion of TNFalpha. The white bar, 10 mum. E Permeabilization was performed to detect the intracellular distribution of TNFalpha, which reflecting the cellular residual TNFalpha. The white bar, 10 mum. F After treated with TiPs for 2 h, p110delta-knockdown RAW264.7 were imaged with confocal microscopy to detect cellular IL-6 distribution. The white bar, 10 mum. G Surface TNFalpha and intracellular TNFalpha were analysed respectively by FCM assay after 2 h TiPs stimulation with or without 10 muM IC87114. For surface TNFalpha detection, permeabilization was not applied and 10 muM TAPI-1 (TACE inhibitor) was employed to block the cl