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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [14]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- NBP1-49643 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49643, RRID:AB_10011762
- Product name
- Rabbit Polyclonal TGN46 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Bovine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.66 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references RAB21 interacts with TMED10 and modulates its localization and abundance.
APEX2-mediated RAB proximity labeling identifies a role for RAB21 in clathrin-independent cargo sorting.
Transient Compound Treatment Induces a Multigenerational Reduction of Oxysterol-Binding Protein (OSBP) Levels and Prophylactic Antiviral Activity.
PERK inhibition attenuates the abnormalities of the secretory pathway and the increased apoptotic rate induced by SIL1 knockdown in HeLa cells.
The Origin, Dynamic Morphology, and PI4P-Independent Formation of Encephalomyocarditis Virus Replication Organelles.
Endoplasmic reticulum-to-Golgi transitions upon herpes virus infection.
The armadillo protein p0071 controls KIF3 motor transport.
DHHC5-mediated palmitoylation of S1P receptor subtype 1 determines G-protein coupling.
Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection.
An essential receptor for adeno-associated virus infection.
The Bif-1-Dynamin 2 membrane fission machinery regulates Atg9-containing vesicle generation at the Rab11-positive reservoirs.
Syntaxin 5-dependent retrograde transport to the trans-Golgi network is required for adeno-associated virus transduction.
CutA divalent cation tolerance homolog (Escherichia coli) (CUTA) regulates β-cleavage of β-amyloid precursor protein (APP) through interacting with β-site APP cleaving protein 1 (BACE1).
ATP7A gene addition to the choroid plexus results in long-term rescue of the lethal copper transport defect in a Menkes disease mouse model.
Del Olmo T, Lacarrière-Keïta C, Normandin C, Jean D, Boisvert FM, Jean S
Biology open 2019 Sep 9;8(9)
Biology open 2019 Sep 9;8(9)
APEX2-mediated RAB proximity labeling identifies a role for RAB21 in clathrin-independent cargo sorting.
Del Olmo T, Lauzier A, Normandin C, Larcher R, Lecours M, Jean D, Lessard L, Steinberg F, Boisvert FM, Jean S
EMBO reports 2019 Feb;20(2)
EMBO reports 2019 Feb;20(2)
Transient Compound Treatment Induces a Multigenerational Reduction of Oxysterol-Binding Protein (OSBP) Levels and Prophylactic Antiviral Activity.
Roberts BL, Severance ZC, Bensen RC, Le AT, Kothapalli NR, Nuñez JI, Ma H, Wu S, Standke SJ, Yang Z, Reddig WJ, Blewett EL, Burgett AWG
ACS chemical biology 2019 Feb 15;14(2):276-287
ACS chemical biology 2019 Feb 15;14(2):276-287
PERK inhibition attenuates the abnormalities of the secretory pathway and the increased apoptotic rate induced by SIL1 knockdown in HeLa cells.
Capone V, Clemente E, Restelli E, Di Campli A, Sperduti S, Ornaghi F, Pietrangelo L, Protasi F, Chiesa R, Sallese M
Biochimica et biophysica acta. Molecular basis of disease 2018 Oct;1864(10):3164-3180
Biochimica et biophysica acta. Molecular basis of disease 2018 Oct;1864(10):3164-3180
The Origin, Dynamic Morphology, and PI4P-Independent Formation of Encephalomyocarditis Virus Replication Organelles.
Melia CE, van der Schaar HM, de Jong AWM, Lyoo HR, Snijder EJ, Koster AJ, van Kuppeveld FJM, Bárcena M
mBio 2018 Apr 17;9(2)
mBio 2018 Apr 17;9(2)
Endoplasmic reticulum-to-Golgi transitions upon herpes virus infection.
Wild P, Kaech A, Schraner EM, Walser L, Ackermann M
F1000Research 2017;6:1804
F1000Research 2017;6:1804
The armadillo protein p0071 controls KIF3 motor transport.
Becher A, Eiseler T, Porzner M, Walther P, Keil R, Bobrovich S, Hatzfeld M, Seufferlein T
Journal of cell science 2017 Oct 1;130(19):3374-3387
Journal of cell science 2017 Oct 1;130(19):3374-3387
DHHC5-mediated palmitoylation of S1P receptor subtype 1 determines G-protein coupling.
Badawy SMM, Okada T, Kajimoto T, Ijuin T, Nakamura SI
Scientific reports 2017 Nov 29;7(1):16552
Scientific reports 2017 Nov 29;7(1):16552
Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection.
Serfass JM, Takahashi Y, Zhou Z, Kawasawa YI, Liu Y, Tsotakos N, Young MM, Tang Z, Yang L, Atkinson JM, Chroneos ZC, Wang HG
The Journal of biological chemistry 2017 Jun 16;292(24):10097-10111
The Journal of biological chemistry 2017 Jun 16;292(24):10097-10111
An essential receptor for adeno-associated virus infection.
Pillay S, Meyer NL, Puschnik AS, Davulcu O, Diep J, Ishikawa Y, Jae LT, Wosen JE, Nagamine CM, Chapman MS, Carette JE
Nature 2016 Feb 4;530(7588):108-12
Nature 2016 Feb 4;530(7588):108-12
The Bif-1-Dynamin 2 membrane fission machinery regulates Atg9-containing vesicle generation at the Rab11-positive reservoirs.
Takahashi Y, Tsotakos N, Liu Y, Young MM, Serfass J, Tang Z, Abraham T, Wang HG
Oncotarget 2016 Apr 12;7(15):20855-68
Oncotarget 2016 Apr 12;7(15):20855-68
Syntaxin 5-dependent retrograde transport to the trans-Golgi network is required for adeno-associated virus transduction.
Nonnenmacher ME, Cintrat JC, Gillet D, Weber T
Journal of virology 2015 Feb;89(3):1673-87
Journal of virology 2015 Feb;89(3):1673-87
CutA divalent cation tolerance homolog (Escherichia coli) (CUTA) regulates β-cleavage of β-amyloid precursor protein (APP) through interacting with β-site APP cleaving protein 1 (BACE1).
Zhao Y, Wang Y, Hu J, Zhang X, Zhang YW
The Journal of biological chemistry 2012 Mar 30;287(14):11141-50
The Journal of biological chemistry 2012 Mar 30;287(14):11141-50
ATP7A gene addition to the choroid plexus results in long-term rescue of the lethal copper transport defect in a Menkes disease mouse model.
Donsante A, Yi L, Zerfas PM, Brinster LR, Sullivan P, Goldstein DS, Prohaska J, Centeno JA, Rushing E, Kaler SG
Molecular therapy : the journal of the American Society of Gene Therapy 2011 Dec;19(12):2114-23
Molecular therapy : the journal of the American Society of Gene Therapy 2011 Dec;19(12):2114-23
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TGN46 Antibody [NBP1-49643] - Analysis of extracts from HeLa cells using TGN46 antibody (NBP1-49643, 1:100).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TGN46 Antibody [NBP1-49643] - Analysis of TGOLN2 in human renal cell carcinoma.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on U-937 cells with NBP1-49643 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with TGN46 Antibody NBP1-49643AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with NBP1-49643C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
