- PA5-23068 - Invitrogen Antibodies TGOLN2 antibody

Submitted references Nitric Oxide Inhibition of Rickettsia rickettsii.

Fitzsimmons LF, Clark TR, Hackstadt T
Infection and immunity 2021 Nov 16;89(12):e0037121

PML nuclear body-residing proteins sequentially associate with HPV genome after infectious nuclear delivery.

Guion L, Bienkowska-Haba M, DiGiuseppe S, Florin L, Sapp M
PLoS pathogens 2019 Feb;15(2):e1007590

The HCMV Assembly Compartment Is a Dynamic Golgi-Derived MTOC that Controls Nuclear Rotation and Virus Spread.

Procter DJ, Banerjee A, Nukui M, Kruse K, Gaponenko V, Murphy EA, Komarova Y, Walsh D
Developmental cell 2018 Apr 9;45(1):83-100.e7

Cellular v-ATPase is required for virion assembly compartment formation in human cytomegalovirus infection.

Pavelin J, McCormick D, Chiweshe S, Ramachandran S, Lin YT, Grey F
Open biology 2017 Nov;7(11)

Human Papillomavirus Major Capsid Protein L1 Remains Associated with the Incoming Viral Genome throughout the Entry Process.

DiGiuseppe S, Bienkowska-Haba M, Guion LGM, Keiffer TR, Sapp M
Journal of virology 2017 Aug 15;91(16)

Incoming human papillomavirus type 16 genome resides in a vesicular compartment throughout mitosis.

DiGiuseppe S, Luszczek W, Keiffer TR, Bienkowska-Haba M, Guion LG, Sapp MJ
Proceedings of the National Academy of Sciences of the United States of America 2016 May 31;113(22):6289-94

Topography of the Human Papillomavirus Minor Capsid Protein L2 during Vesicular Trafficking of Infectious Entry.

DiGiuseppe S, Keiffer TR, Bienkowska-Haba M, Luszczek W, Guion LG, Müller M, Sapp M
Journal of virology 2015 Oct;89(20):10442-52

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of TGN46 in extracts from HeLa cells. Samples were incubated in TGN46 polyclonal antibody (Product # PA5-23068) using a dilution of 1:100.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using TGN46 Polyclonal Antibody (Product # PA5-23068) and a 39 kDa band corresponding to TGN46 was observed across cell lines and tissues tested along with an uncharacterized band (*) at ~80 kDa. Modified whole cell extracts (30 µg lysate) of HeLa (Lane 1), T98G (Lane 2), Mouse muscle (Lane 3), Rat muscle (Lane 4), Mouse skin (Lane 5) and Rat skin (Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (PA5-23068, 1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Trans-Golgi Network 46 using a polyclonal antibody (Product # PA5-23068).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of TGN46 in HepG2 cells fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. Samples were incubated in TGN46 polyclonal antibody (Product # PA5-23068) using a dilution of 5 µg/mL for 1 hour at room temperature. Antibody conjugated to Alexa Fluor 488. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of TGN46 in A549 cells. Samples were incubated in TGN46 polyclonal antibody (Product # PA5-23068) followed by Alexa Fluor 488-conjugated Goat to rabbit IgG secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of TGN46 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TGN46 Polyclonal Antibody (Product # PA5-23068) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of TGN46 in human renal cell carcinoma. Samples were incubated in TGN46 polyclonal antibody (Product # PA5-23068).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of TGN46 in U-937 cells. Samples were incubated in TGN46 polyclonal antibody (Product # PA5-23068) and a matched isotype control using a dilution of 1 µg/mL for 30 minutes at room temperature followed by rabbit IgG APC-conjugated secondary antibody. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of TGN46 in HepG2 cells. Samples were incubated in TGN46 polyclonal antibody (Product # PA5-23068) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to DyLight 650.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4. ATP6V0C is required for HCMV virion assembly compartment biogenesis. Fibroblast cells were transfected with ATP6V0C siRNA or negative control siRNA and at 72 h post-transfection were infected with AD169. At 144 hpi cells were fixed, permeabilised and stained for early endosomes or trans-Golgi vacuoles, (EEA1:green or TGN46:green), viral tegument protein (pp28:red) and nuclei (DAPI:blue). Images were acquired on a Zeiss LSM710 confocal microscope. Images presented here are maximum-intensity projections compiled from multiple 0.33 um slices through the z -axis.

PA5-23068

Antibody data