PA5-36571

antibody from Invitrogen Antibodies

Targeting: RPL7 humL7-1, L7

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36571

Provider

Invitrogen Antibodies

Product name

RPL7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 29 kDa. Purity is >95% by SDS-PAGE.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Ribosome impairment regulates intestinal stem cell identity via ZAKɑ activation.

Silva J, Alkan F, Ramalho S, Snieckute G, Prekovic S, Garcia AK, Hernández-Pérez S, van der Kammen R, Barnum D, Hoekman L, Altelaar M, Zwart W, Suijkerbuijk SJE, Bekker-Jensen S, Faller WJ
Nature communications 2022 Aug 2;13(1):4492

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPL7 in Lane 1: HCT116 whole cell lysate (10 µg), Lane 2: MCF-7 whole cell lysate (10 µg), Lane 3: the Brain tissue lysate of mouse (10 µg), Lane 4: the Brain tissue lysate of rat (10 µg). Samples were incubated with RPL7 polyclonal antibody (Product # PA5-36571) at a dilution of 1:5,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPL7 using RPL7 polyclonal antibody (Product # PA5-36571) at a dilution of 1:500. Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: H9C2 whole cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPL7 in Lane 1: SK-OVCAR3 whole cell lysate (40 µg), Lane 2: MCF-7 whole cell lysate (40 µg), Lane 3: L02 whole cell lysate (40 µg), Lane 4: the Spleen tissue lysate of rat (40 µg), Lane 5: the Spleen tissue lysate of mouse (40 µg). Samples were incubated with RPL7 polyclonal antibody (Product # PA5-36571) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPL7 in Lane 1: HCT116 whole cell lysate (10 µg), Lane 2: MCF-7 whole cell lysate (10 µg), Lane 3: the Brain tissue lysate of mouse (10 µg), Lane 4: the Brain tissue lysate of rat (10 µg). Samples were incubated with RPL7 polyclonal antibody (Product # PA5-36571) at a dilution of 1:5,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of RPL7 in HEK293T cell lysate (sepharose bead conjugate), (lane 2 and lane 3) and Nonspecific IgG Control (sepharose bead conjugate, lane 4 and lane 5). Lane 1 is 30% input. Samples were incubated with RPL7 polyclonal antibody (Product # PA5-36571) Results legends show supernatant (upward arrow) and deposition (downward arrow).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Metabolism is translationally regulated in fetal-like ISCs. a Experimental workflow of the RiboSeq experiment comparing the translatome of Rptor fl/fl organoids to WT. Crypt cultures were generated from VillinCre ERT2 RPL22.HA mice both in the presence and absence of Raptor. Tagged ribosomes were pulldown using an HA antibody and ribosome protected fragments were sequenced and mapped to the genome. b Western blot analysis confirming efficient pulldown of HA-tagged RPL22 ribosomes. RPL7 co-immunoprecipitation is used as an indicator of intact ribosomes. beta-actin serves as a loading control. Experiments were done in one biological replicate. c Gene Set Enrichment Analysis based on differential translation efficiency data comparing Rptor fl/fl organoids to stem cell enriched cultures ( n = 3). Enrichment is shown for transcriptional signatures related to oxidative phosphorylation and glycolysis. p values were determined using the clusterProfiler package. d Experimental workflow of the RiboSeq experiment comparing the translatome of intestinal stem cells from Rptor fl/fl to WT mice. Ribosomes were tagged specifically in the instestinal stem cells of mice using an Lgr5Cre ERT2 promoter, both in the presence and absence of Raptor. 24 h after tamoxifen induction, the ribosomes are recombined with the HA tag exclusively in the intestinal stem cells, allowing the capture and further study of their translatome by RiboSeq. e Imunohistochemistry staining of Lgr5Cre ERT2 RPL22.HA intest