Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A300-740A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse RPL7 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RPL7 antibody BL2443 (Product # A300-740A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate (B). RPL7 was also immunoprecipitated by rabbit anti-RPL7 antibody BL2444 (Product # A300-741A). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse RPL7 by WB (h&m). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg), and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RPL7 antibody BL2443 (Cat. No. A300-740A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). RPL7 was also immunoprecipitated by rabbit anti-RPL7 antibody BL2444 (Cat. No. A300-741A). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
