Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Other assay [1]
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Validation data
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- Product number
- MA1-16746 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STEP Monoclonal Antibody (23E5)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: rat brain.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 23E5
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5.
Brigidi GS, Santyr B, Shimell J, Jovellar B, Bamji SX
Nature communications 2015 Sep 3;6:8200
Nature communications 2015 Sep 3;6:8200
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 5 PSD-95 enhances the binding and tyrosine phosphorylation of DHHC5 by Fyn kinase. ( a - d ) Fourteen DIV hippocampal neurons were stimulated, lysed at the indicated time points, immunoprecipitated with anti- ( a ) DHHC5 or ( c ) Fyn and blots probed with the indicated antibodies. ( a , b ) DHHC5 tyrosine phosphorylation (phY; P =0.007, F 6,14 =7.749) and DHHC5/Fyn interactions ( P